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Abstract

Introduction

Although is considered a normal human microbiota, it may cause life-threatening infections such as septicemia, urinary tract infections, and enteric infections. Moreover, multidrug-resistant strains are a serious challenge in the clinic due to high mortality rates and the limited number of therapeutic options. Hence, the current study aimed to benefit from as a source of green synthesis of copper nanoparticles (Cu-NPs), to investigate the antibacterial features against multidrug-resistant and to measure the cytotoxicity of Cu-NPs.

Methods

were used as a reducing agent for Cu-NP synthesis, and then XRD, zeta potential, UV-Vis, FTIR, SEM, and DLS analyses were performed to characterize the synthesized NPs. Moreover, the MIC and zone of inhibition values of Cu-NPs were measured and compared to common antibiotics. Additionally, the MTT assay was performed to assess the cytotoxicity.

Results

The green synthesized Cu-NPs were spherical and uniform with a size of ~200 nm. The MIC of Cu-NPs was 1024 μg/ml on the MDR strain of , representing the antibacterial activity comparable to levofloxacin (p-value>0.05) but less than imipenem and trimethoprim (p-value<0.001). Moreover, the CC50 of synthesized Cu-NPs was 731.2 μg/ml and significantly lower than the studied antibiotics (p-value<0.001).

Conclusion

The findings may suggest Cu-NPs as a promising antibacterial strategy against MDR strains of , however, further studies are encouraged to clarify the safety of optimized doses.

© 2025 Bentham Science Publishers
© 2025 The Author(s). Published by Bentham Science Publishers. This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2025-06-13
2025-09-13

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The Green Synthesis of Cu Nanoparticles and Investigation of the Antibacterial Properties and Cytotoxicity on Multidrug-Resistant E. coli
Bentham Science Publishers ; https://doi.org/10.2174/0115680266379834250605135159
/content/journals/ctmc/10.2174/0115680266379834250605135159
/content/journals/ctmc/10.2174/0115680266379834250605135159
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  • Article Type:     Research Article
Keywords: bacteria ; Multi-drug resistant ; therapy ; E. coli ; nanoparticle

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