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image of Total Synthesis of Biologically Potent Peptides and their In Silico Studies: A TAG Approach

Abstract

Introduction

Current trends in peptide synthesis protocols have emerged as the most attractive domain in the field of pharma and medicine. Since most of the peptide/peptidomimetic-based molecules serve as potential candidates for many diseases, as they are bioavailable molecules.

Methods

We present the synthesis of bioactive peptides through TAGGING approach with the help of TAG-OH as a linker to the -protected amino acid.

Results

FRDEHKK and NKDRG are two peptides that possess antioxidant and antiproliferative activity, and their investigations reveal that they exhibit anticancer properties when bound to the AXL kinase and EGFR proteins.

Discussion

This TAG method enables the easy isolation of peptides at each step as solids, and all the impurities were washed off by simple filtration. The method allows a bulk-scale preparation of the peptides without any difficulty, and hence the protocol is highly efficient for the production of peptides of therapeutic importance.

Conclusion

The two peptides FRDEHKK and NKDRG were isolated as fine solids with 82% and 85% yield and were characterized by NMR and MASS spectroscopy. studies reveal FRDEHKK and NKDRG peptides exhibit good affinity towards EGFR and AXL kinase.

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2025-08-04
2026-01-31

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Total Synthesis of Biologically Potent Peptides and their In Silico Studies: A TAG Approach
Bentham Science Publishers ; https://doi.org/10.2174/0115680266377804250721145504
/content/journals/ctmc/10.2174/0115680266377804250721145504
/content/journals/ctmc/10.2174/0115680266377804250721145504
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  • Article Type:     Research Article
Keywords: Anticancer ; Antioxidant ; TAGGING technique ; NKDRG peptide ; Bioactive peptides ; FRDEHKK

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