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image of The Therapeutic Potentials of Chemo-Herbal Combination: Enhancing Anti-tumor immunity and Anti-cancer activity against Ehrlich Ascites Carcinoma

Abstract

Introduction

The combinational chemo-herbal therapy effectively suppresses tumors and reduces their chemoresistance.

Objective

This study evaluated the anti-cancer and anti-tumor immunity properties of a chemo-herbal therapy combination of Curcumin (Cur), Ginger (Gin), Clove (Clov), Ginger (Gin), and Amygdalin (Amyg) with Doxorubicin (DOX) against the Ehrlich Ascites Carcinoma (EAC) cell line.

Methods

The study examined the anti-tumor effects of herbal extracts from Cur, Gin, Clov, and Amyg alone and with DOX against EAC using the MTT assay. It evaluated anti-cancer and anti-tumoral immunity, cell counts, growth rates, and apoptosis of EAC cells, phenotypic expression of T lymphocytes (CD4+T and CD8+T) and natural killer (NK) cells, splenocyte and leucocyte counts, as well as Liver and kidney functions in EAC-challenged mice treated with extracts from Cur, Gin, Clov, and Amyg alone and with DOX.

Results

Chemo-herbal therapy using extracts of Cur, Gin, Clov, and Amyg combined with DOX showed significant anti-proliferative effects on EAC . , this combined treatment in tumor-challenged mice reduced EAC cell proliferation by decreasing cell counts and increasing apoptosis rates. Furthermore, it enhanced the expression of CD4+T and CD8+T lymphocytes and NK cells, while slightly increasing total leukocyte counts, neutrophils, and monocytes, but reducing total lymphocytes and eosinophils. Additionally, the combination therapy mitigated EAC-induced liver and kidney damage, restoring normal organ function.

Discussion

The integration of herbal extracts derived from Cur, Gin, Clov, and Amyg with the chemotherapy drug DOX may improve the effectiveness of chemotherapy in combating tumors and strengthen the immune response of the host against cancer.

Conclusion

Chemo-herbal therapy combining Cur, Gin, Clov, and Amyg with DOX may improve anti-tumor effects, enhance immune response, and reduce chemotherapy side effects, but requires additional studies for validation.

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2026-01-22
2026-01-31

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/content/journals/ctmc/10.2174/0115680266374075251103144517
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The Therapeutic Potentials of Chemo-Herbal Combination: Enhancing Anti-tumor immunity and Anti-cancer activity against Ehrlich Ascites Carcinoma
Bentham Science Publishers ; https://doi.org/10.2174/0115680266374075251103144517
/content/journals/ctmc/10.2174/0115680266374075251103144517
/content/journals/ctmc/10.2174/0115680266374075251103144517
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  • Article Type:     Research Article
Keywords: EAC ; Anti-cancer ; Anti-tumor immunity ; Chemotherapy ; DOX ; Herbal extracts

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