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2000
Volume 25, Issue 22
  • ISSN: 1568-0266
  • E-ISSN: 1873-4294

Abstract

Aim

To investigate the antimicrobial potential of methanol fruit extract of against Methicillin-Resistant (MRSA) and elucidate its mode of action.

Background

The rise of antimicrobial resistance (AMR) demands the exploration of alternative therapeutic strategies to combat resistant pathogens.

Objective

To evaluate the efficacy of methanol extract against MRSA, and identify its active constituents and mechanism of action.

Methods

The fruits of were extracted using various solvents, with hexane and methanol yielding the highest results. Microbroth dilution assays assessed antimicrobial activity, while assays such as Alamar blue, Scanning Electron Microscopy (SEM), protein, and nucleic acid leakage examined metabolic disruption and cell membrane integrity. Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify active compounds, and molecular docking studies assessed interactions with key MRSA proteins.

Results

The methanol extract demonstrated significant antimicrobial activity against MRSA, causing metabolic disruption and leakage of cellular contents as evidenced by various assays including alarm blue, SEM, and protein and nucleic acid leakage assay. GC-MS analysis identified alpha-linoleic acid and palmitic acid as key active components. Molecular docking studies confirmed their inhibition of beta-lactamase activity, cell wall biosynthesis, efflux pumps, and virulence factors.

Conclusion

The findings suggest that methanol fruit extract has promising potential as a natural remedy against AMR associated with MRSA.

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Chemical Profiling and Antibacterial Potential of Methanol Extract of Solanum xanthocarpum Fruits against Methicillin-Resistant Staphylococcus aureus: Implications for AMR Management
Curr. Top. Med. Chem. 25, 2666 (2025); https://doi.org/10.2174/0115680266364747250223154131
/content/journals/ctmc/10.2174/0115680266364747250223154131
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  • Article Type:     Research Article
Keyword(s): alpha-linoleic acid and palmitic acid; Antibacterial; Gas Chromatography-Mass Spectrometry (GC-MS); Methicillin-Resistant Staphylococcus aureus; Molecular docking

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