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2000
Volume 12, Issue 1
  • ISSN: 2215-0838
  • E-ISSN: 2215-0846
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Abstract

Introduction

A Polyherbal Unani Powder Formulation for Urinary Tract Infections (PUFP-UTI) comprises L., L., , L., and L. This study aimed to evaluate the physicochemical characteristics, phytochemical composition, quantification of luteolin using HPLC, microbial safety, and antibacterial activity to assess the quality, efficacy, and safety of the formulation.

Methods

Physicochemical analysis included organoleptic properties, ash value, density, moisture content, and extractive values. Phytochemical screening detected phenols, tannins, flavonoids, alkaloids, glycosides, saponins, terpenoids, and sterols. Quantitative analysis measured total phenols, tannins, flavonoids, and alkaloids. Luteolin content was quantified by HPLC. Microbial contamination was assessed following WHO guidelines. Antibacterial activity was tested against , , , , , , and using the agar well diffusion method.

Results

PUFP-UTI exhibited acceptable physicochemical properties and contained significant amounts of phenols, tannins, flavonoids, alkaloids, and luteolin. The formulation met WHO safety standards for microbial contamination. It also showed substantial antibacterial activity against all tested pathogens. These results indicate that PUFP-UTI is a microbiologically safe herbal formulation with notable antimicrobial efficacy.

Discussion

The presence of multiple phytoconstituents, such as flavonoids and tannins, along with measurable luteolin content, may contribute to the observed antibacterial effect of PUFP-UTI. Its broad-spectrum activity against common uropathogens supports its pharmacological relevance. The formulation also complies with basic quality and safety benchmarks, reinforcing its potential as a safe traditional remedy.

Conclusion

PUFP-UTI's antibacterial properties support its traditional use in treating urinary tract infections. Further stability studies and clinical trials are recommended to confirm its therapeutic potential.

© 2026 The Author(s). Published by Bentham Science Publishers.
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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In-vitro Antimicrobial Profiling and Standardization of a Classical Unani Polyherbal Formulation Targeting Uropathogens
Current Traditional Medicine 12, E22150838408522 (2026); https://doi.org/10.2174/0122150838408522250815080237
/content/journals/ctm/10.2174/0122150838408522250815080237
/content/journals/ctm/10.2174/0122150838408522250815080237
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  • Article Type:     Research Article
Keyword(s): antimicrobial activity; E. coli; HPLC fingerprinting; polyherbal Unani formulation; standardization; Unani medicine; Urinary tract infection

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