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2000
Volume 11, Issue 2
  • ISSN: 2215-0838
  • E-ISSN: 2215-0846

Abstract

Background

Several drugs have been proposed for the treatment of breast cancer, but none has fully treated the disease so far.

Methods

MCF7 cells were cultured in RPMI-1640 medium, consisting of different concentrations of aqueous-alcoholic extract of the fruit of (0.125, 0.25, 0.5, 1, 1.5, 2, 2.5, and 5 mg/mL) for 48 and 72 hours. MTT assay was used to determine the cell proliferation inhibition (IC). The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V/PI apoptosis detection kit. RT-PCR method was carried out to assess the fold changes of OCT4, NANOG, and SOX2 genes. Two-way ANOVA (tukeys) and t-test (repeated measure) were used for the statistical analysis of obtained data MTT assay and RT-PCR method, respectively. < 0.05 was considered significant.

Results

Our results have shown that cell death was induced by increasing fruit of extract concentration. IC was observed at 48 h culture period with 4.81 mg/mL, and 72 h with 2.72 mg/mL fruit extract, respectively. Flow cytometry results exhibited an obviously significant augmentation in apoptotic MF7 cells. According to RT-PCR findings on the fruit of extract-treated cells, the mean expression of OCT4, NANOG, and SOX2 genes decreased after 48 and 72 h of incubation with IC concentration compared to controls.

Conclusion

The fruit of the species was able to decrease the expression of self-renewal genes in the MCF7 cell line. Therefore, the fruit of extract can be considered a promising candidate for the management of human breast cancer after clinical trials.

© 2025 Bentham Science Publishers
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The Survey of the Fruit of Capparis spinosa Extracts Effects on the Expression Profile of Essential Self-renewal Genes in MCF7 Cell Line
Current Traditional Medicine 11, E051023221795 (2025); https://doi.org/10.2174/0122150838258062230920091457
/content/journals/ctm/10.2174/0122150838258062230920091457
/content/journals/ctm/10.2174/0122150838258062230920091457
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  • Article Type:     Research Article
Keyword(s): anticancer agent; Breast cancer; fruit of Capparis spinosa; MCF-7 cell line; NANOG; OCT4; SOX2

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