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2000
Volume 20, Issue 4
  • ISSN: 1574-888X
  • E-ISSN: 2212-3946

Abstract

Background

Periodontitis is a common complication of diabetes, with advanced glycation end products (AGEs) playing a key role in its pathogenesis. Albiflorin, a monoterpene glycoside, has shown potential anti-inflammatory and antioxidant properties. This study aims to investigate the effects of albiflorin on AGEs-induced gingival fibroblasts and its underlying mechanisms.

Objective

This study aimed to evaluate the role of albiflorin in mitigating ROS production, inflammation, and MMP-1 expression in AGEs-induced gingival fibroblasts.

Methods

The viability of gingival fibroblasts treated with albiflorin and AGEs was assessed using CCK-8 assays. ROS levels were measured by DCF staining, and the expression of inflammatory markers and MMP-1 was evaluated by ELISA and qPCR. The involvement of the NF-κB and Nrf2 pathways was examined by immunoblotting.

Results

Albiflorin enhanced the viability of AGEs-induced gingival fibroblasts and reduced ROS production. It also decreased the expression of IL-6, IL-8, RAGE, and MMP-1, suggesting an anti-inflammatory effect. Mechanistically, albiflorin modulated the NF-κB and Nrf2 pathways in AGEs-induced gingival fibroblasts.

Conclusion

Albiflorin exhibited protective effects against AGEs-induced oxidative stress and inflammation in gingival fibroblasts, highlighting its potential as a therapeutic agent for periodontitis in diabetic patients. The modulation of the NF-κB and Nrf2 pathways by albiflorin provides insight into its mechanism of action.

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2024-04-27
2025-09-06

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/content/journals/cscr/10.2174/011574888X302174240420165019
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Albiflorin Inhibits Advanced Glycation End Products-Induced ROS and MMP-1 Expression in Gingival Fibroblasts
Curr Stem Cell Res Ther 20, 434 (2025); https://doi.org/10.2174/011574888X302174240420165019
/content/journals/cscr/10.2174/011574888X302174240420165019
/content/journals/cscr/10.2174/011574888X302174240420165019
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  • Article Type:     Research Article
Keyword(s): advanced glycation end products (AGEs); albiflorin; Gingival fibroblast; NF-κB; Nrf2; ROS

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