Syntheses and Effect of Bombesin-Fragment6-14 and its Four Analogues on Food Intake in Rats

A nonapeptide, H-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2 , corresponding to amino acids 6 to 14 of bombesin and its four analogues were synthesized by a solid-phase method and were tested for their comparative effect on reducing activity on food intake using male Wistar rats. The synthetic bombesin-fragment6- 14 showed reducing effect on food intake using male Wistar rats. Of the synthetic analogues, [Phe(4F)13 ]bombesin-fragment6-14 exhibited the most potent effect. The reducing effect on food intake of [Sar 11 ]bombesin-fragment6-14 was lower than that of our synthetic bombesin-fragment6-14, but the other two analogues, [beta Ala11]bombesin-fragment6-14 and [1-Nal8 ]bombesin-fragment6-14 showed no reducing effect on food intake in rats.