Current Pharmaceutical Analysis - Volume 8, Issue 4, 2012

A simple reverse-phase (RP) high performance liquid chromatography (HPLC) method was developed and validated, according to the International Harmonisation Guidelines (ICH), for the determination of risperidone (RISP) from solid lipid nanoparticles (SLN). Chromatographic runs were performed on a RP-C18 column, using an isocratic mobile phase of methanol, acetate buffer (0.05 M; pH 4.6) and triethylamine (60:40:0 Read More

Cocaine and crack usage is a major public health concern worldwide, mainly when the target consumers are pregnant women. The purpose of this study was to develop and validate a cost-effective method for identification and quantification of cocaine, benzoylecgonine, cocaethylene, and anhydroecgonine methyl ester in meconium samples, using solid-phase extraction (SPE) and GC/MS (ion trap – full scan mode) to be em Read More

In this paper, a rapid and effective chromatographic procedure for determining the curcumin encapsulation efficiency in poly(lactic-co-glycolic acid) (PLGA) and poly(lactic-co-glycolic acid)-polyethyleneglycol (PLGA-PEG) nanoparticles via reversed-phase high-performance liquid chromatography (RP-HPLC) using a fluorescence detector and low flow rate is described. Chromatographic runs were performed on a RP C18 column (25 Read More

The construction and response characteristics of sensors based on polyvinyl chloride used for the assay of ciprofloxacin (CFX) were described. The electroactive compound was the complex between ciprofloxacin and tetraphenylborate. The wider linear concentration range 2x10-2 - 1.97x10-5mol/L was achieved when dibutylphthalate (DBP) and bis(2-ethylhexyl)sebacate (DES) were used as plasticizers, with slopes of 29.12 an Read More

Electrochemical behavior of zofenopril (ZOF) was studied via experimental electrochemical methods and theoretical calculations performed at B3LYP/6-31+G(d)//AM1 level. Optimum conditions for quantitative determination were investigated by several electrochemical methods such as cyclic voltammetry, square-wave voltammetry and bulk electrolysis. Electrochemical parameters like charge transfer, diffusion and surf Read More

The HPLC-UV method previously developed for the analysis of the flavonoid 2”-O-rhamnosylswertisin (I), an active compound of dried leaf extracts of Aleurites moluccana L. Willd, was fully validated with the inclusion of another flavonoid, swertisin (II). The stability-indicating capability of the method was established by analyzing forced degradation extract samples (acid, alkali, neutral, oxidative and photolytic condition) in whi Read More

A stability-indicating LC method for the determination of the antihyperglycemic agent sitagliptin phosphate in tablets was developed and validated using the Plackett-Burman experimental design for robustness evaluation and determination of the system suitability limits. Analytical parameters were studied according to International Conference on Harmonization (ICH) guidelines. The analytical column was operated with a soluti Read More

The present work reports a stability-indicating reversed phase ion pair liquid chromatography method for quantitative determination of metformin hydrochloride (MTF) (drug and tablets) and assessment of the cytotoxicity of degraded MTF. Chromatographic separation was performed on a C18 (Phenomenex® Luna), 5.0 μm (250 mm x 4.6 mm) column using isocratic elution. The optimized mobile phase consisted of 10 mmol L-1 Read More

Outpatient parenteral antimicrobial therapy (OPAT) is an important clinical tool in the treatment of severe infections away from the hospital setting. The stability of amoxicillin sodium in elastomeric infusion devices has been determined for parenteral antimicrobial therapy in the outpatient setting. Firstly, stability was studied at either ambient room temperature (25°C ±1), or at refrigerated temperature (4°C). The practice f Read More

A new stability-indicating RP-HPLC method for the determination of metformin hydrochloride and nateglinide in tablets was developed. The chromatographic separation was performed on a C18 column employing 0.05 M potassium dihydrogen orthophosphate (pH 3)- acetonitrile (28:72 v/v) as the mobile phase with UV detection at 216 nm. Under the proposed chromatographic conditions, favourable retention parameter Read More

Chemical degradation of drugs often results in altered therapeutic efficacy and can lead to toxic side effects. A single, short and sensitive GC-ECD method for the quantification of degradation and process related impurities of lindane has been developed and validated in all of its pharmaceutical topical dosage forms (shampoo, cream and lotion). Three major degradants were observed during forced degradation ( Read More

This article describes the development and optimization of a UV spectrophotometric method to evaluate the isomers of citral in cyclodextrins complexes by a partial least squares (PLS) regression model. The central composite design (CCD) associated with the response surface methodology (RSM) was applied to select the wavelength range that provides the best prediction results. β-cyclodextrin (β-CD) and hydr Read More

The degradation kinetics of cefuroxime lysine in aqueous solution were investigated by a developed liquid chromatograph/mass spectrometry (LC/MS) method with pH ranging from 1.5 to 11.0. The influences of pH, temperature, ionic strength, irradiation and oxidation on the degradation rate were evaluated, and the corresponding kinetic parameters were calculated. The decomposition of cefuroxime lysine was observed Read More

This paper describes the analysis of abamectin and the characterization of its degradation products in formulation. A fast and selective reversed-phase HPLC was developed and validated for the quantitation of degradation products. Two major degradation products observed in stress study samples were isolated and characterized using chromatography and high resolution FT-MS. Abamectin and its degradation products pre Read More

Due to the difficulties in plasma concentration measurement of lisinopril, it is of utmost importance to develop a new accurate analysis method for this drug. A randomized, double-blind, two-period, two-group crossover design was conducted to scrutinize the bioequivalence of a lisinopril generic product. After administration of test or reference products to each volunteer, the active ingredient was determined in plasma samples u Read More