Current Proteomics - Volume 3, Issue 4, 2006

Since protein phosphorylation was accepted to play a key role in cellular metabolism the investigation of this important posttranslational modification has gained ever-growing interest of researchers. However, until recently, technical challenges have made large scale studies on protein phosphorylation impossible. With dramatic improvements in both phosporylated peptides/proteins enrichment methods and in th Read More

Since translation is a central process in all living organisms, the components of translational machinery containing aminoacyltRNA synthetases, initiation, elongation, and releasing factors and ribosomal proteins have been considered as housekeepers of the cells. While these components are necessary for translational control, many of them have been found also to be involved in the control of cell fate through the diverse fun Read More

Signal transduction through reversible protein phosphorylation plays a central role in cellular physiology in both normal and disease states, and mass spectrometry is well-suited to the task of identifying and cataloging protein phosphorylation sites. Recent advances in sample enrichment strategies, instrumentation, and software availability allow researchers to identify hundreds of phosphorylation sites in a single study. Ho Read More

Molecular dynamics (MD) simulation methods have been an effective source of generating biomolecular-level structural information in immunology, as feedback to understand basic science and to design new experiments, leading to the discovery of drugs and vaccines. Different soluble or surface-bound proteins secreted by immune cells exchange signals through the formation of specialized molecular complexes. Molecule Read More

A surface plasmon resonance (SPR) biosensor technology has recently been applied biochemically and clinically to the study of immunologic recognition and the evaluation of binding parameters for various interactions between antibodies (Abs) and antigens (Ags) at liquid-solid interface. The simple interaction between hapten and Ab fragment, e.g., variable single-chain fragment and antigenbinding fragment, can be desc Read More