Current Proteomics - Volume 3, Issue 2, 2006

Proteomics has become a powerful tool facilitating hypothesis-driven exploration of disease states as well as offering a global approach which can uncover potentially important, though unexpected links to disease etiology. Despite many recent advances, the pathophysiological basis of Alzheimer's disease is incompletely understood. Alzheimer's dementia almost certainly results from a constellation of cellular changes, Read More

Protein phosphorylation, a reversible post-translational modification, has been long identified as a critical regulator in a growing number of cellular processes. Most notably, phosphorylation has been implicated in the signal transduction pathways regulating the cell cycle, cellular proliferation, differentiation, and gene expression events. The ubiquitous nature of phosphorylation as a control mechanism has led to the study of ph Read More

A major goal of proteomics is the complete description of all the proteins present in cells, tissues and biological fluids. The method of choice for identifying and characterizing proteins for such purposes is protease digestion coupled with mass spectrometry (MS) and subsequent protein sequence database searching. New software tools to increase the sensitivity and specificity of MS based protein identification and meth Read More

Living organisms are composed of millions different kinds of molecules. Both hydrophilic and hydrophobic molecules make up cells and tissues. However, in healthy tissues, hydrophobic portions (hyppos) are seldom exposed on the surface of the biological molecules and supramolecular organization. Since the water-insoluble molecules could form non-productive and even toxic aggregates in aqueous body fluid, they hav Read More