Current Proteomics - Volume 3, Issue 1, 2006

In recent years, protein cross-linking technology using appropriate chemical cross-linking reagents and identification of cross-linked peptides and proteins by mass spectrometry has been used successfully to elucidate the intra- and inter-molecular interactions of protein molecules. Identification of the cross-linking sites within a single polypeptide chain or across polypeptide chains of a protein can provide insight into Read More

Lung cancer is a challenging clinical problem worldwide and it is the leading cause of death from cancer for both men and women. Because of this, the ability to diagnose lung cancer in its early stages is considered crucial to achieve decreased lung cancer mortality. Proteomics has recently been introduced to the field of cancer research, and its potential applications are just beginning to be understood. Unlike the study of a s Read More

This review deals with chemical approaches that facilitate proteomics research. The methods include chemical cleavage of peptide bonds, stepwise degradation of proteins, and site-specific modification enabling the discrimination between functional groups. These reactions could enhance the efficacy of mass spectrometry (MS) for identification, quantification, and sequencing of peptides and proteins. Particular stress is laid on Read More

The rapid development of proteomics has been made possible primarily by the progress in analytical and preparative separation methods. Biological systems are so complex that the separation procedures employed must be highly efficient as well as highly selective. The latter requirement is best met by affinity separations based on molecular recognition. The present review critically discusses the properties of Read More