Studies of Technological Parameters Influencing the Protein-Polymeric Nanoparticles Adsorption Process for Transmucosal Administration

Aim of this work was to study the technological parameters influencing the adsorption process of Bovine Serum Albumin (used as model protein) on the surface of PLGA (polylactic co-glycolic acid) based nanoparticles. Several factors as adsorption mediums, concentration ratio between proteins and nanoparticles, temperature, time of incubation were evaluated and main importance was done to the chemical characteristics of polymers and surfactants used for nanoparticles obtainment. Afterward adsorption studies, further tests of permeation through cellular monolayers and synthetic membranes were done in order to study the desorption process of BSA from nanoparticles surface. The mediums influence on the desorption rate of the protein from the nanoparticles and the influence of polymeric excipients on the permeation profile of the protein were evaluated. Data showed demonstrated that the adsorption process as well as the desorption capability and the permeation rate of BSA are strictly influenced by the nanoparticles composition and several kind of interactions between polymers and protein.