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2000
Volume 3, Issue 2
  • ISSN: 2666-8629
  • E-ISSN: 2666-8637

Abstract

Aims

Hypertension is one of the chronic diseases causing a serious health problem in many countries worldwide. Therefore, the prevention and amelioration of hypertension with medicinal food is considered to be important for maintaining good health and well-being. The main objective of this study is to verify that the extract of lotus seed has a significant inhibitory effect on the angiotensin I-converting enzyme (ACE).

Background

Lotus (Nelumbo nucifera) is well known to have a variety of biological and medical activities and is popularly used as a traditional medicinal food in several East Asian countries. In a previous study, we reported that the aqueous extract of lotus root used as a popular foodstuff caused an inhibitory effect on the angiotensin I-converting enzyme (ACE), suggesting its possible effectiveness for improving high blood pressure.

Objective

The purpose of the present study was to reveal the effect of lotus seed extract on ACE activity and to identify a potentially active component of the extract originating from lotus seed.

Methods

The ACE inhibitory activity was determined using Hippuryl-L-histidyl-L-leucin (HHL) as substrate, and the amount of hippuric acid formed enzymatically was determined using an HPLC system.

Results

The lotus seed extract was shown to strongly inhibit the ACE activity, thus ameliorating the hypertensive state. Furthermore, the fractionation of the lotus seed extract provided evidence for suggesting nicotianamine as a putative ACE-inhibiting component contained in the extract.

Conclusion

These findings were considered to propose the possibility that the lotus seed and the lotus root might benefit patients with hypertension.

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2025-09-08

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References

  1. LewingtonS. ClarkeR. QizilbashN. PetoR. CollinsR. Age-specific relevance of usual blood pressure to vascular mortality: A meta-analysis of individual data for one million adults in 61 prospective studies.Lancet200236093491903191310.1016/S0140‑6736(02)11911‑8 12493255
     [Google Scholar]
  2. RanillaL.G. KwonY.I. ApostolidisE. ShettyK. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America.Bioresour. Technol.2010101124676468910.1016/j.biortech.2010.01.093 20185303
     [Google Scholar]
  3. SinghP. MishraA. SinghR. GoswamiS. SinghA. TiwariK.D. Hypertension and herbal plant for its treatment: A review.Indian J Res Pharm Biotechnol20153358366
     [Google Scholar]
  4. AtlasS.A. The renin-angiotensin aldosterone system: Pathophysiological role and pharmacologic inhibition.J. Manag. Care Pharm.200713892010.18553/jmcp.2007.13.s8‑b.9 17970613
     [Google Scholar]
  5. WeirM. DzauV.J. The renin-angiotensin-aldosterone system: A specific target for hypertension management.Am. J. Hypertens.199912420521310.1016/S0895‑7061(99)00103‑X 10619573
     [Google Scholar]
  6. IwaniakA. MinkiewiczP. DarewiczM. Food-originating ACE inhibitors, including antihypertensive peptides, as preventive food components in blood pressure reduction.Compr. Rev. Food Sci. Food Saf.201413211413410.1111/1541‑4337.12051 33412648
     [Google Scholar]
  7. MaquedaM.D. MirallesB. RecioI. LedesmaH. Antihypertensive peptides from foodproteins:A review.Food Funct.2012335036110.1039/c2fo10192k 22249830
     [Google Scholar]
  8. MirallesB. AmigoL. RecioI. Critical review and perspectives on food-derived antihypertensive peptides.J. Agric. Food Chem.201866369384939010.1021/acs.jafc.8b02603 30130403
     [Google Scholar]
  9. AlukoR.E. Antihypertensive peptides from food proteins.Annu. Rev. Food Sci. Technol.20156123526210.1146/annurev‑food‑022814‑015520 25884281
     [Google Scholar]
  10. DikmenD.C. YucetepeA. GulerK.F. DaskayaH. OzcelikB. Angiotensine-I-converting enzyme (ACE)-inhibitory peptides from plants.Nutrients20179431610.3390/nu9040316 28333109
     [Google Scholar]
  11. NorrisR FitzGeraldJ Richard Antihypertensive peptides from food proteins.Bioactive food peptides in health and disease.2013457210.5772/51710
     [Google Scholar]
  12. Al ShukorN. Van CampJ. GonzalesG.B. Angiotensin-converting enzyme inhibitory effects by plant phenolic compounds: A study of structure activity relationships.J. Agric. Food Chem.20136148118321183910.1021/jf404641v 24219111
     [Google Scholar]
  13. BalasuriyaN.B.W. RupasingheV.H.P. Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension.Funct. Food Health Dis.20111517218810.31989/ffhd.v1i5.132
     [Google Scholar]
  14. DubiosM.A.L. FrankU. WangerH. Search for potential angiotensine converting enzyme (ACE)-inhibitors from plants.Phytomedicine2001814752
     [Google Scholar]
  15. HafezA. AskariH. KhanN. AkramM. HanifS. UroojN. Molecular modering of plant flavonoid as angiotensine converting enzyme (ACE) inhibitors in hypertension: A docking study.World J Phermaceutical Sci20142422519
     [Google Scholar]
  16. ClemensS. DeinleinU. AhmadiH. HörethS. UraguchiS. Nicotianamine is a major player in plant Zn homeostasis.Biometals201326462363210.1007/s10534‑013‑9643‑1 23775667
     [Google Scholar]
  17. CurieC. CassinG. CouchD. Metal movement within the plant: Contribution of nicotianamine and yellow stripe 1-like transporters.Ann. Bot.2009103111110.1093/aob/mcn207 18977764
     [Google Scholar]
  18. SchulerM. BauerP. Heavy metals need assistance: The contribution of nicotianamine to metal circulation throughout the plant and the arabidopsis NAS gene family.Front Plant Sci201126910.3389/fpls.2011.00069 22639605
     [Google Scholar]
  19. ScholzG. BeckerR. PichA. StephanU.W. Nicotianamine ‐ A common constituent of strategies I and II of iron acquisition by plants: A review.J. Plant Nutr.199215101647166510.1080/01904169209364428
     [Google Scholar]
  20. HayashiA. KimotoK. Nicotianamine preferentially inhibits Angiotensin I-converting enzyme.J. Nutr. Sci. Vitaminol.200753433133610.3177/jnsv.53.331 17934238
     [Google Scholar]
  21. HayashiA. KimotoK. Studies on the mechanism of antihypertensive action by nicotianamine.J. Nutr. Sci. Vitaminol.201056424224610.3177/jnsv.56.242 20924146
     [Google Scholar]
  22. NishiboriN. SagaraT. MoritaK. Identification of nicotianamine as a potential inhibitor of angiotensine I-converting enzyme in aqueous extract of okara dry powder.J. Food Nutr. Res.2021602161167
     [Google Scholar]
  23. ShimizuE. HayashiA. TakahashiR. AoyagiY. MurakamiT. KimotoK. Effects of angiotensin I-converting enzyme inhibitor from Ashitaba (Angelica keiskei) on blood pressure of spontaneously hypertensive rats.J. Nutr. Sci. Vitaminol.199945337538310.3177/jnsv.45.375 10524357
     [Google Scholar]
  24. TakenakaT. MurayamaT. FurushoT. TakenakaY. Antihypertensive Effects of Nicotianamine from Soybean Broth in Spontaneously Hypertensive Rats.Food Sci. Technol. Res.200915554154610.3136/fstr.15.541
     [Google Scholar]
  25. TowatanaH.N. ChaiyamuttiP. PanthongK. MahabusarakamW. RukachaisirikulV. Antioxidative and free radical scavenging activities of some plants used in Thai folk medicine.Pharm. Biol.200644322122810.1080/13880200600685592
     [Google Scholar]
  26. JungH.A. KimJ.E. ChungH.Y. ChoiJ.S. Antioxidant principles ofNelumbo nucifera stamens.Arch. Pharm. Res.200326427928510.1007/BF02976956 12735685
     [Google Scholar]
  27. KuoY.C. LinY.L. LiuC.P. TsaiW.J. Herpes simplex virus type 1 propagation in HeLa cells interrupted by Nelumbo nucifera.J. Biomed. Sci.20051261021103410.1007/s11373‑005‑9001‑6 16132118
     [Google Scholar]
  28. SinhaS. MukherjeeP.K. MukherjeeK. PalM. MandalS.C. SahaB.P. Evaluation of antipyretic potential ofNelumbo nucifera stalk extract.Phytother. Res.200014427227410.1002/1099‑1573(200006)14:4<272::AID‑PTR556>3.0.CO;2‑H 10861971
     [Google Scholar]
  29. SridharK.R. BhatR. Lotus – A potential nutraceutical source.Agric. Technol. Thail.20073143155
     [Google Scholar]
  30. ZhangY. LuX. ZengS. Nutritional composition, physiological functions and processing of lotus (Nelumbo nucifera Gaertn.) seeds: A review.Phytochem. Rev.201514332133410.1007/s11101‑015‑9401‑9
     [Google Scholar]
  31. NishiboriN. SagaraT. HiroiT. Inhibition of angiotensin I-converting enzyme (ACE-I) by aqueous extracts prepared from edible and non-edible parts of lotus root.Phytopharmacol20123309318
     [Google Scholar]
  32. GoudaM.K.G. GowdaL.R. RaoA.G.A. PrakashV. Angiotensin I-converting enzyme inhibitory peptide derived from glycinin, the 11S globulin of soybean (Glycine max).J. Agric. Food Chem.200654134568457310.1021/jf060264q 16786999
     [Google Scholar]
  33. ChenY.H. ChenH.Y. HsuC.L. YenG.C. Induction of apoptosis by the Lactuca indica L. in human leukemia cell line and its active components.J. Agric. Food Chem.20075551743174910.1021/jf063118t 17295517
     [Google Scholar]
  34. KakeiY. YamaguchiI. KobayashiT. A highly sensitive, quick and simple quantification method for nicotianamine and 2′-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants.Plant Cell Physiol.200950111988199310.1093/pcp/pcp141 19880395
     [Google Scholar]
  35. WadaY. YamaguchiI. TakahashiM. NakanishiH. MoriS. NishizawaN.K. Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.Biosci. Biotechnol. Biochem.200771243544110.1271/bbb.60496 17284859
     [Google Scholar]
  36. CheugH.S. WangF.L. OndettiM.A. SaboE.F. CushmaD.W. Binding of peptide substrate and inhibitors of angiotensine-converting enzyme.J. Biol. Chem.198025540140710.1016/S0021‑9258(19)86187‑2
     [Google Scholar]
  37. FanH. LiaoW. WuJ. Molecular interactions, bioavailability, and cellular mechanisms of angiotensin-converting enzyme inhibitory peptides.J. Food Biochem.2019431e1257210.1111/jfbc.12572 31353484
     [Google Scholar]
  38. KhanT.H.M. DedachiK. MatsuiT. Dipeptide inhibitors of thermolysin and angiotensin I-converting enzyme.Curr. Top. Med. Chem.201212161748176210.2174/1568026611209061748 23030610
     [Google Scholar]
  39. IbrahimH.R. AhmedA.S. MiyataT. Novel angiotensin-converting enzyme inhibitory peptides from caseins and whey proteins of goat milk.J. Adv. Res.201781637110.1016/j.jare.2016.12.002 28053783
     [Google Scholar]
  40. YangY. MarczakE.D. YokooM. UsuiH. YoshikawaM. Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach Rubisco.J. Agric. Food Chem.200351174897490210.1021/jf026186y 12903942
     [Google Scholar]
  41. TonouchiH. SuzukiM. UchidaM. OdaM. Antihypertensive effect of an angiotensin converting enzyme inhibitory peptide from enzyme modified cheese.J. Dairy Res.200875328429010.1017/S0022029908003452 18620614
     [Google Scholar]
/content/journals/cff/10.2174/0126668629301379240507042304
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Nicotianamine as a Potential Active Component of Lotus Seed Extract for Inhibiting Angiotensin-converting Enzyme
Curr Func Foods 3, E310524230584 (2025); https://doi.org/10.2174/0126668629301379240507042304
/content/journals/cff/10.2174/0126668629301379240507042304
/content/journals/cff/10.2174/0126668629301379240507042304
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  • Article Type:     Research Article
Keyword(s): ACE inhibition; antihypertensive effect; chronic disease; hypertension; Lotus seed; nicotianamine

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