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image of Rapid and Comprehensive Identification of Vincosamide Metabolites in vitro and in vivo in Rats by Ultra-High Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap-High Resolution Mass Spectrometry

Abstract

Background

Vincosamide, an indole alkaloid extracted from , exhibits a range of pharmacological activities, such as anti-tumor, antibacterial, and anti-inflammatory properties. However, despite its promising therapeutic applications, there is a notable gap in research focused on the metabolic pathways of vincosamide.

Objective

This study aims to investigate the metabolism of vincosamide both and in rats, and to elucidate its metabolic pathways.

Methods

Samples of liver microsomal incubation, plasma, bile, urine, and feces following vincosamide administration were analyzed by ultra-high performance liquid chromatography-quadrupole-Exactive Orbitrap-high resolution mass spectrometry (UHPLC-Q-Exactive Orbitrap HRMS). The collected data were analyzed using Compound Discovery 3.2 software and the molecular network method. The metabolites identified through these methodologies were subsequently validated using Xcalibur 4.1 software, which provided information on retention times, parent ions, and characteristic fragment ions.

Results

A total of 37 metabolites were identified, including 8 and 32 (3 in plasma, 7 in bile, 22 in urine, and 17 in feces). While the metabolism of vincosamide differs and in rats, the type of metabolic reaction that occurs is well-defined. The predominant metabolic pathways are oxidation, reduction, deglycosylation, hydration, glucuronidation, methylation, sulfation, glycine conjugation, cysteine conjugation, taurine conjugation, and complex reactions.

Conclusion

This study elucidates the metabolism of vincosamide and in rats, thereby expanding the metabolite profile of vincosamide. These findings provide a foundation for the potential development of new drugs based on vincosamide.

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2025-04-16
2025-09-15

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Rapid and Comprehensive Identification of Vincosamide Metabolites in vitro and in vivo in Rats by Ultra-High Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap-High Resolution Mass Spectrometry
Bentham Science Publishers ; https://doi.org/10.2174/0113892002351614250401153450
/content/journals/cdm/10.2174/0113892002351614250401153450
/content/journals/cdm/10.2174/0113892002351614250401153450
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  • Article Type:     Research Article
Keywords: Compound Discovery ; UHPLC-Q-Exactive Orbitrap HRMS ; Vincosamide ; molecular network ; metabolic pathway ; metabolite

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