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image of The Effect of Different Extraction Method and Validation of the HPLC Method for Sinensetin Quantification in Cat Whiskers (Orthosiphon aristatus Blume Miq.)

Abstract

Introduction

The study investigates the quantification of sinensetin, a bioactive compound with antioxidant, anti-obesity, and anti-cancer properties, in cat's whiskers (Orthosiphon aristatus). The primary aim is to evaluate the effect of different extraction methods on sinensetin yield and to validate an high-performance liquid chromatography (HPLC) method for accurate quantification.

Methods

The research employed two extraction techniques: reflux and maceration. The HPLC method was validated for accuracy, precision, linearity, and specificity using a reverse-phase C18 column maintained at 25°C. The mobile phase consisted of acetonitrile (60:40) and 0.1% formic acid, with an isocratic elution. A 20 µl injection volume and a flow rate of 1 ml/min were used for analysis.

Results

Validation results indicated a recovery rate of 98.37 ± 3.44% and a precision relative standard deviation (RSD) of 1.12%. The linearity correlation value was found to be 0.999. Sinensetin concentrations were quantified at 0.42 ± 0.006% for the macerated extract and 0.30 ± 0.006% for the reflux extract.

Discussion

The findings demonstrate that the HPLC method is both accurate and precise for determining sinensetin levels, with maceration yielding higher concentrations than reflux. These results contribute to the understanding of extraction efficiency and the potential therapeutic applications of sinensetin.

Conclusion

This study confirms that accurate quantification of sinensetin in cat's whiskers is achievable using validated HPLC methods, highlighting the importance of extraction techniques in maximising bioactive compound yields.

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2025-07-16
2025-10-31

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The Effect of Different Extraction Method and Validation of the HPLC Method for Sinensetin Quantification in Cat Whiskers (Orthosiphon aristatus Blume Miq.)
Bentham Science Publishers ; https://doi.org/10.2174/0122127968385340250630183912
/content/journals/ccb/10.2174/0122127968385340250630183912
/content/journals/ccb/10.2174/0122127968385340250630183912
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  • Article Type:     Research Article
Keywords: validation ; sinensetin ; Orthosiphon aristatus (Blume) Miq. purple variety ; high-performance liquid chromatography (HPLC)

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