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image of Isolation and Characterization of Several Halophilic Bacteria from Marine Environments

Abstract

Introduction

Marine environments harbor diverse microbial communities that have evolved to thrive under extreme conditions. Among these, halophilic and halotolerant bacteria are of particular interest due to their ability to produce ectoine, a compatible solute with valuable biotechnological applications, especially in cosmetics, medicine, and stress-protective formulations.

Methods

Marine water samples were collected and subjected to serial dilution and culturing techniques to isolate halophilic and halotolerant bacterial strains. A total of 20 distinct bacterial isolates were obtained. These isolates were screened for their tolerance to high salt concentrations (≥2M NaCl) and elevated temperatures (≥35°C). Morphological and biochemical characteristics were assessed, and selected isolates underwent 16S rRNA gene sequencing for taxonomic identification.

Results

Out of the 20 isolates, 14 demonstrated the ability to grow at ≥2M NaCl and at temperatures of 35°C or higher. Morphological and biochemical analyses identified six dominant genera, including , , and . Molecular characterization confirmed that isolate KR-30 was , showing 99.73% sequence similarity to JCM 2479.

Discussion

The high salt and temperature tolerance of the isolates, particularly , indicates their potential for industrial applications where such stress conditions are common. The dominance of halophilic genera suggests a promising source of robust microbial candidates for ectoine production and other biotechnological processes.

Conclusion

This study highlights the potential of marine-derived halophilic and halotolerant bacteria, especially , for ectoine production under extreme conditions. These findings provide a solid foundation for future research and development of marine microbial resources for industrial biotechnology.

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2025-10-30
2026-02-25

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/content/journals/cbiot/10.2174/0122115501425807251017044247
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Isolation and Characterization of Several Halophilic Bacteria from Marine Environments
Bentham Science Publishers ; https://doi.org/10.2174/0122115501425807251017044247
/content/journals/cbiot/10.2174/0122115501425807251017044247
/content/journals/cbiot/10.2174/0122115501425807251017044247
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  • Article Type:     Research Article
Keywords: Halophilic bacteria ; salt tolerance ; biotechnological applications ; temperature tolerance ; marine microbiology ; Marinococcus halophilus ; ectoine production ; compatible solutes

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