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image of Application of UPLC-Q-Exactive-MS Technology Combined With Network Pharmacology to Investigate the Mechanism of Action of Aster Tataricus L. Extract in the Treatment of Drug-induced Liver Injury
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Abstract

Objective/Introduction

To investigate the pharmacodynamic material basis and potential mechanism of Aster tataricus L. total flavonoids (ATF) in treating isoniazid (INH) and rifampicin (RIF)-induced drug-induced liver injury (DILI) in C57BL/6 mice

Methods

ATF was extracted using ultrasonic extraction and purified with AB-8 macroporous adsorbent resin. A DILI model was established in male C57BL/6 mice using INH and RIF. Histopathological changes were assessed by HE staining, and serum levels of ALT and AST, as well as liver levels of SOD, MDA, GSH-Px, and T-AOC, were measured. UPLC-Q-Exactive-MS was used to analyze ATF and serum components in DILI mice, followed by network pharmacology analysis and molecular docking validation.

Results

TThe extraction rate of ATF was 31.9%, and the purification rate was 83.1%. ATF treatment alleviated cell necrosis and inflammation, reduced organ index, and normalized biochemical indices, with the best effects observed at low doses. UPLC-Q-Exactive-MS identified 15 blood-entry components, 9 prototype components, and 4 core targets with high binding energies.

Discussion

Efficient extraction and purification methods for total flavonoids from Aster tataricus L. were developed. Their pharmacological basis and potential targets for treating DILI were identified, providing a theoretical basis for DILI treatment.

Conclusion

This study provides a theoretical basis for the treatment of DILI using ATF. The developed extraction and purification methods efficiently obtained ATF, and its pharmacological basis and potential targets were identified. This research offers a new direction for exploring natural products from Traditional Chinese Medicine for DILI treatment.

© 2025 The Author(s). Published by Bentham Science Publishers
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode.
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2025-05-05
2025-09-09

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Application of UPLC-Q-Exactive-MS Technology Combined With Network Pharmacology to Investigate the Mechanism of Action of Aster Tataricus L. Extract in the Treatment of Drug-induced Liver Injury
Bentham Science Publishers ; https://doi.org/10.2174/0115734110348076250428113339
/content/journals/cac/10.2174/0115734110348076250428113339
/content/journals/cac/10.2174/0115734110348076250428113339
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  • Article Type:     Research Article
Keywords: network pharmacology ; molecular docking ; Extraction ; Aster tataricus L. total flavonoids ; drug-induced liver injury ; purification

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