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image of Analytical Techniques for the Quantification and Validation of Resveratrol: A Review

Abstract

Introduction

Trans-resveratrol is a bioactive polyphenol that has been widely studied for its antioxidant, anti-inflammatory, and chemoprotective properties. It holds promise in pharmaceutical and nutraceutical formulations but is limited by poor bioavailability and stability.

Methods

This review synthesizes validated analytical methods for quantifying trans-resveratrol across various matrices. A comprehensive literature search (2000–2024) was conducted using PubMed, Scopus, and Google Scholar, focusing on RP-HPLC, HPTLC, GC, and UV spectroscopy. Method validation follows ICH guidelines.

Results

Thirty-seven validated analytical methods were reviewed. RP-HPLC using C18 columns with acetonitrile-water mobile phases dominated the literature. The most sensitive technique identified was LC-MS/MS (LOD = 0.001 μg/mL), particularly effective in biological samples. Matrix types included wine, serum, and nanoparticle formulations.

Discussion

RP-HPLC and LC-MS/MS have emerged as robust techniques for resveratrol quantification due to their sensitivity and specificity. Emerging tools like biosensors and UPLC offer rapid analysis with lower solvent consumption. Challenges such as isomerization, photodegradation, and matrix interferences necessitate stringent sample-handling protocols.

Conclusion

Advanced chromatographic methods, especially RP-HPLC and LC-MS/MS, are essential for the reliable quantification of trans-resveratrol. Future research should focus on analytical standardization and the development of novel delivery systems to enhance resveratrol's pharmacokinetic profile.

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2025-09-23
2025-09-25

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Analytical Techniques for the Quantification and Validation of Resveratrol: A Review
Bentham Science Publishers ; https://doi.org/10.2174/0118715230403013250901204059
/content/journals/aiaamc/10.2174/0118715230403013250901204059
/content/journals/aiaamc/10.2174/0118715230403013250901204059
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  • Article Type:     Review Article
Keywords: Reverse phase HPLC ; HPTLC ; gas chromatography ; UV spectroscopy ; LC-MS/MS

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