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2000
Volume 24, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Aim

The growing problem of antibiotic-resistant bacteria in clinical environments has driven increased interest in bacteriophage therapy, where viruses specifically target and destroy harmful bacteria. This research explores the therapeutic potential of bacteriophages isolated to combat multi-drug-resistant , using larvae as an experimental model. susceptibility to multiple antibiotics was assessed using the double agar overlay technique. Additionally, the survival rate of the larvae was analyzed to determine the phages' capacity to combat bacterial infection in . Bacteriophage therapy was employed to treat moth wax larvae that were infected with Multidrug-resistant

Introduction

Resistance to commonly used antibiotics has been steadily increasing over the past few decades, and it has been observed to vary depending on the alternative method employed, such as bacteriophage therapy.

Materials and Methods

The study focused on the strain PP31, a Multidrug-Resistant Gram Negative bacterium that was obtained from biomedical waste at ICF Hospital in Tamil Nadu, India. larvae infected with this multidrug-resistant strain of were employed for treatment using bacteriophage.

Results

It was found that a single phage might infect a particular strain of bacteria in the host. It was demonstrated that MDR infected larvae must be treated with a single specific phage dose (20 µL, 104 PFU/mL) at 6 – hour intervals in order to achieve a 95% survival rate for research. By counting the number of germs in the larvae, the results were confirmed.

Conclusion

Our research shows that although phages were shown to be highly contagious , specific phage dosages were required for effective treatment in living animals.

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2025-05-05
2026-03-09

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The Potential Therapeutic Advantages of Bacteriophage Targeting MDR Pseudomonas aeruginosa using the Infection Model of Galleria mellonella
Anti Infect. Agents 24, E22113525380554 (2026); https://doi.org/10.2174/0122113525380554250418072309
/content/journals/aia/10.2174/0122113525380554250418072309
/content/journals/aia/10.2174/0122113525380554250418072309
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  • Article Type:     Research Article
Keyword(s): antibiotic resistance; bacteriophage therapy; Galleria mellonella; moth wax larvae; multi-drug-resistant; Pseudomonas aeruginosa

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