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2000
Volume 24, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Introduction/Background

Chalcone-based compounds display potential antifungal activity. They are considered to be versatile pharmacophores that can be utilized for the designing and development of numerous bioactive lead compounds. In this work, some new chalcone derivatives were synthesized, which are expected to possess antifungal activity, making them potentially useful for treating fungal infections.

Objective

The rate at which fungi develop resistance to antifungals is outpacing the discovery of new antifungal classes. Developing new compounds with the same behavior but improved pharmacological action has proven to be a significant challenge for scientists. Therefore, discovering novel drugs is crucial for combating health problems and enhancing the quality of human life. For this reason, in this study, we synthesized new chalcones and evaluated their antifungal activity.

Methods

In the initial phase, studies were employed to predict the physicochemical properties and biological activities of chalcone derivatives. A new series of chalcones was synthesized using synthetic methodologies, and characterization techniques, such as spectroscopy and chromatography, were employed to confirm the identity and purity of the compounds. The antifungal potential of chalcones was checked by the zone inhibition method (Kirby-Bauer method) using and strains. Subsequently, molecular docking against fungal targets was performed to elucidate the binding interactions, providing valuable insights into the structure-activity relationship, and cytotoxicity studies were performed to assess the potential toxicity of these compounds.

Results and Discussion

The target compounds (Ca-Ce) were successfully synthesized and characterized by spectroscopic and analytical methods. The findings from the computational properties and activity against fungal stains like and revealed that the newly synthesized chalcones exhibit significant antifungal potential.

Conclusion

The newly synthesized chalcones, especially Cc and Ce, exhibited antifungal activity nearly equivalent to that of standard drugs.

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References

  1. XuM. WuP. ShenF. JiJ. RakeshK.P. Chalcone derivatives and their antibacterial activities: Current development.Bioorg. Chem.20199110313310.1016/j.bioorg.2019.103133 31374524
     [Google Scholar]
  2. KantR. KumarD. AgarwalD. GuptaR.D. TilakR. AwasthiS.K. AgarwalA. Synthesis of newer 1,2,3-triazole linked chalcone and flavone hybrid compounds and evaluation of their antimicrobial and cytotoxic activities.Eur. J. Med. Chem.2016113344910.1016/j.ejmech.2016.02.041 26922227
     [Google Scholar]
  3. SinghP. AnandA. KumarV. Recent developments in biological activities of chalcones: A mini review.Eur. J. Med. Chem.20148575877710.1016/j.ejmech.2014.08.033 25137491
     [Google Scholar]
  4. SinghM. KaurM. SilakariO. Flavones: An important scaffold for medicinal chemistry.Eur. J. Med. Chem.20148420623910.1016/j.ejmech.2014.07.013 25019478
     [Google Scholar]
  5. QinH.L. ZhangZ.W. LekkalaR. AlsulamiH. RakeshK.P. Chalcone hybrids as privileged scaffolds in antimalarial drug discovery: A key review.Eur. J. Med. Chem.202019311221510.1016/j.ejmech.2020.112215 32179331
     [Google Scholar]
  6. LeiteF.F. SousaD.N.F. OliveiraD.B.H.M. DuarteG.D. FerreiraM.D.L. ScottiM.T. FilhoJ.M.B. RodriguesL.C. MouraD.R.O. Mendonça-JuniorF.J.B. ScottiL. Anticancer activity of chalcones and its derivatives: Review and in silico studies.Molecules20232810400910.3390/molecules28104009 37241750
     [Google Scholar]
  7. NowakowskaZ. A review of anti-infective and anti-inflammatory chalcones.Eur. J. Med. Chem.200742212513710.1016/j.ejmech.2006.09.019 17112640
     [Google Scholar]
  8. RashidU.H. XuY. AhmadN. MuhammadY. WangL. Promising anti-inflammatory effects of chalcones via inhibition of cyclooxygenase, prostaglandin E2, inducible NO synthase and nuclear factor κb activities.Bioorg. Chem.20198733536510.1016/j.bioorg.2019.03.033 30921740
     [Google Scholar]
  9. KumarA. GuptaV. SinghS. GuptaY.K. Synthesis of some chalcone and their heterocyclic derivatives as potential antimicrobial agents: A review.Asian J. Res. Chem201710222523910.5958/0974‑4150.2017.00038.4
     [Google Scholar]
  10. MelloD.M.V.P. Abrahim-VieiraB.A. DomingosT.F.S. JesusD.J.B. SousaD.A.C.C. RodriguesC.R. SouzaA.M.T. A comprehensive review of chalcone derivatives as antileishmanial agents.Eur. J. Med. Chem.201815092092910.1016/j.ejmech.2018.03.047 29602038
     [Google Scholar]
  11. HayatF. MoseleyE. SalahuddinA. ZylV.R.L. AzamA. Antiprotozoal activity of chloroquinoline based chalcones.Eur. J. Med. Chem.20114651897190510.1016/j.ejmech.2011.02.004 21377771
     [Google Scholar]
  12. CasanoG. DumètreA. PannecouqueC. HutterS. AzasN. RobinM. Anti-HIV and antiplasmodial activity of original flavonoid derivatives.Bioorg. Med. Chem.201018166012602310.1016/j.bmc.2010.06.067 20638854
     [Google Scholar]
  13. MittalA. VashisthaV.K. DasD.K. Recent advances in the antioxidant activity and mechanisms of chalcone derivatives: A computational review.Free Radic. Res.2022565-637839710.1080/10715762.2022.2120396 36063087
     [Google Scholar]
  14. IqbalH. PrabhakarV. SangithA. ChandrikaB. BalasubramanianR. Synthesis, anti-inflammatory and antioxidant activity of ring-A-monosubstituted chalcone derivatives.Med. Chem. Res.201423104383439410.1007/s00044‑014‑1007‑z
     [Google Scholar]
  15. ChoudharyN.A. KumarA. JuyV. Design, synthesis and evaluation of chalcone derivatives as anti-inflammatory, antioxidant and antiulcer agents.Lett. Drug Des. Discov.20129547948810.2174/157018012800389368
     [Google Scholar]
  16. HuangT. LiX. MaierM. O’Brien-SimpsonN.M. HeathD.E. O’ConnorA.J. Using inorganic nanoparticles to fight fungal infections in the antimicrobial resistant era.Acta Biomater.2023158567910.1016/j.actbio.2023.01.019 36640952
     [Google Scholar]
  17. LópezS.N. CastelliM.V. ZacchinoS.A. DomínguezJ.N. LoboG. Charris-CharrisJ. CortésJ.C.G. RibasJ.C. DeviaC. RodríguezA.M. EnrizR.D. In vitro antifungal evaluation and structure–activity relationships of a new series of chalcone derivatives and synthetic analogues, with inhibitory properties against polymers of the fungal cell wall.Bioorg. Med. Chem.2001981999201310.1016/S0968‑0896(01)00116‑X 11504637
     [Google Scholar]
  18. PrasadY.R. RaoA.L. RambabuR. Synthesis and antimicrobial activity of some chalcone derivatives.J. Chem.20085346146610.1155/2008/876257
     [Google Scholar]
  19. TsuchiyaH. SatoM. AkagiriM. TakagiN. TanakaT. IinumaM. Anti-candida activity of synthetic hydroxychalcones.Pharmazie19944910756758 7816901
     [Google Scholar]
  20. SatoM. TsuchiyaH. AkagiriM. FujiwaraS. FujiiT. TakagiN. MatsuuraN. IinumaM. Growth inhibitory properties of chalcones to Candida.Lett. Appl. Microbiol.1994181535510.1111/j.1472‑765X.1994.tb00800.x
     [Google Scholar]
  21. ReddyN.P. AparoyP. ReddyT.C.M. AchariC. SridharP.R. ReddannaP. Design, synthesis, and biological evaluation of prenylated chalcones as 5-LOX inhibitors.Bioorg. Med. Chem.201018165807581510.1016/j.bmc.2010.06.107 20667741
     [Google Scholar]
  22. LahtchevK.L. BatovskaD.I. ParushevS.P. UbiyvovkV.M. SibirnyA.A. Antifungal activity of chalcones: A mechanistic study using various yeast strains.Eur. J. Med. Chem.200843102220222810.1016/j.ejmech.2007.12.027 18280009
     [Google Scholar]
  23. CabreraM. SimoensM. FalchiG. LavaggiM.L. PiroO.E. CastellanoE.E. VidalA. AzquetaA. MongeA. CeráinD.A.L. SagreraG. SeoaneG. CerecettoH. GonzálezM. Synthetic chalcones, flavanones, and flavones as antitumoral agents: Biological evaluation and structure–activity relationships.Bioorg. Med. Chem.200715103356336710.1016/j.bmc.2007.03.031 17383189
     [Google Scholar]
  24. DuckiS. RennisonD. WooM. KendallA. ChabertJ.F.D. McGownA.T. LawrenceN.J. Combretastatin-like chalcones as inhibitors of microtubule polymerization. Part 1: Synthesis and biological evaluation of antivascular activity.Bioorg. Med. Chem.200917227698771010.1016/j.bmc.2009.09.039 19837593
     [Google Scholar]
  25. XiaY. YangZ.Y. XiaP. BastowK.F. NakanishiY. LeeK.H. Antitumor agents. Part 202: Novel 2′-amino chalcones: Design, synthesis and biological evaluation.Bioorg. Med. Chem. Lett.200010869970110.1016/S0960‑894X(00)00072‑X 10782667
     [Google Scholar]
  26. DainaA. MichielinO. ZoeteV. SwissADME: A free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules.Sci. Rep.2017714271710.1038/srep42717 28256516
     [Google Scholar]
  27. WiniwarterS. RidderströmM. UngellA.L. AnderssonT.B. ZamoraI. Use of molecular descriptors for absorption, distribution, metabolism, and excretion predictions.Comprehensive Medicinal Chemistry.United StatesElsevier200753155410.1016/B0‑08‑045044‑X/00140‑1
     [Google Scholar]
  28. KumarB.R.P. SoniM. BhikhalalU.B. KakkotI.R. JagadeeshM. BommuP. NanjanM.J. Analysis of physicochemical properties for drugs from nature.Med. Chem. Res.201019898499210.1007/s00044‑009‑9244‑2
     [Google Scholar]
  29. ChristensonJ.C. KorgenskiE.K. RelichR.F. Laboratory diagnosis of infection due to bacteria, fungi, parasites, and rickettsiae. Principles and Practice of Pediatric Infectious Diseases.United StatesElsevier20181422143410.1016/B978‑0‑323‑40181‑4.00286‑3
     [Google Scholar]
  30. GautamA.K. VermaR.K. AvasthiS. Sushma, BohraY. DevadathaB. NiranjanM. SuwannarachN. Current insight into traditional and modern methods in fungal diversity estimates.J. Fungi20228322610.3390/jof8030226
     [Google Scholar]
  31. SainiP. RehmanZ.U. KumarS. Synthesis and biological evaluation of some new chalcone derivatives as anti-inflammatory agents.Curr. Drug Discov. Technol.2023201e13062220591010.2174/1570163819666220613153225 35702807
     [Google Scholar]
  32. SainiP. KumarS. Computational studies and synthesis of new heterocyclics as CNS agents.Curr. Drug Discov. Technol.2024213e18092322119510.2174/1570163820666230918100218 37723630
     [Google Scholar]
  33. SilvaL.N. MelloD.T.P. RamosS.D.L. BranquinhaM.H. SantosD.A.L.S. Current challenges and updates on the therapy of fungal infections.Curr. Top. Med. Chem.201919749549910.2174/156802661907190531093808 31210103
     [Google Scholar]
  34. Espinel-IngroffA. Arthington-SkaggsB. IqbalN. EllisD. PfallerM.A. MesserS. RinaldiM. FothergillA. GibbsD.L. WangA. Multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin B, and caspofungin.J. Clin. Microbiol.20074561811182010.1128/JCM.00134‑07 17428932
     [Google Scholar]
  35. SinghJ. SinghJ. ZamanM. GuptaA.K. Evaluation of microdilution and disk diffusion methods for antifungal susceptibility testing of dermatophytes.Med. Mycol.200745759560210.1080/13693780701549364 17885958
     [Google Scholar]
  36. BenoudaH. BouchalB. ChalliouiA. OulmidiA. HaritT. MalekF. RiahiA. BellaouiM. BouammaliB. Synthesis of a series of chalcones and related flavones and evaluation of their antibacterial and antifungal activities.Lett. Drug Des. Discov.20181619310010.2174/1570180815666180404130430
     [Google Scholar]
  37. TratratC. HarounM. XenikakisI. LiarasK. TsolakiE. EleftheriouP. PetrouA. AldhubiabB. AttimaradM. VenugopalaK.N. HarshaS. ElsewedyH.S. GeronikakiA. SokovićM. Design, synthesis, evaluation of antimicrobial activity and docking studies of new thiazole-based chalcones.Curr. Top. Med. Chem.201919535637510.2174/1568026619666190129121933 30706816
     [Google Scholar]
  38. Bala Yaswanth KumarS. BantupalliS. AtluriD. Evaluation of selected flavonoids for the anthelmintic and skeletal muscle relaxant activity using animal models.J. Drug Deliv. Ther.202111612713410.22270/jddt.v11i6.5070
     [Google Scholar]
  39. Studies on the Mechanism of Action of the Bioflavonoid Hesperidin Methyl Chalcone in Causing Vascular and Intestinal Smooth Muscle to Relax;M.S. Thesis, University of the Pacific, Department of Physiology and PharmacologyAvailable from: https://scholarlycommons.pacific.edu/uop_etds/1556/ 1964
     [Google Scholar]
  40. ChengP. YangL. HuangX. WangX. GongM. Chalcone hybrids and their antimalarial activity.Arch. Pharm.20203534190035010.1002/ardp.201900350 32003489
     [Google Scholar]
  41. DomínguezJ.N. LeónC. RodriguesJ. Gamboa de DomínguezN. GutJ. RosenthalP.J. Synthesis and evaluation of new antimalarial phenylurenyl chalcone derivatives.J. Med. Chem.200548103654365810.1021/jm058208o 15887974
     [Google Scholar]
  42. OuyangY. LiJ. ChenX. FuX. SunS. WuQ. Chalcone derivatives: Role in anticancer therapy.Biomolecules202111689410.3390/biom11060894 34208562
     [Google Scholar]
  43. KarthikeyanC. MoorthyN.N.S.H. RamasamyS. VanamU. ManivannanE. KarunagaranD. TrivediP. Advances in chalcones with anticancer activities.Rec. Patents Anti. Drug Discov.20141019711510.2174/1574892809666140819153902
     [Google Scholar]
  44. ZhaiL. ChenM. BlomJ. TheanderT.G. ChristensenS.B. KharazmiA. The antileishmanial activity of novel oxygenated chalcones and their mechanism of action.J. Antimicrob. Chemother.199943679380310.1093/jac/43.6.793 10404318
     [Google Scholar]
  45. LiuM. WilairatP. CroftS.L. TanA.L.C. GoM.L. Structure–activity relationships of antileishmanial and antimalarial chalcones.Bioorg. Med. Chem.200311132729273810.1016/S0968‑0896(03)00233‑5 12788347
     [Google Scholar]
  46. TurkovicN. IvkovicB. Kotur-StevuljevicJ. TasicM. MarkovićB. VujicZ. Molecular docking, synthesis and anti-HIV-1 protease activity of novel chalcones.Curr. Pharm. Des.202026880281410.2174/1381612826666200203125557 32013827
     [Google Scholar]
  47. SashidharaK.V. AvulaS.R. MishraV. PalnatiG.R. SinghL.R. SinghN. ChhonkerY.S. SwamiP. BhattaR.S. palitG. Identification of quinoline-chalcone hybrids as potential antiulcer agents.Eur. J. Med. Chem.20158963865310.1016/j.ejmech.2014.10.068 25462272
     [Google Scholar]
  48. JamalH. AnsariW.H. RizviS.J. Evaluation of chalcones – A flavonoid subclass, for, their anxiolytic effects in rats using elevated plus maze and open field behaviour tests.Fundam. Clin. Pharmacol.200822667368110.1111/j.1472‑8206.2008.00639.x 19049672
     [Google Scholar]
  49. RayA. AayilliathK.A. BanerjeeS. ChakrabartiA. DenningD.W. Burden of serious fungal infections in India.Open Forum Infect. Dis.2022912ofac60310.1093/ofid/ofac603
     [Google Scholar]
  50. ZhengY. WangX. GaoS. MaM. RenG. LiuH. ChenX. Synthesis and antifungal activity of chalcone derivatives.Nat. Prod. Res.201529191804181010.1080/14786419.2015.1007973 25675372
     [Google Scholar]
  51. KumarS. KumarA. Synthesis, computational and pharmacological evaluation of 7-(2-(2- (3- (Substituted Phenyl) Acryloyl) Phenoxy) Ethoxy)-4-Methyl-2H-chromen-2-ones as CNS agents.Cent. Nerv. Syst. Agents Med. Chem.2023231576410.2174/1871524923666230130134501 36717998
     [Google Scholar]
  52. BanerjeeP. EckertA.O. SchreyA.K. PreissnerR. ProTox-II: A webserver for the prediction of toxicity of chemicals.Nucleic Acids Res.201846W1W257W26310.1093/nar/gky318 29718510
     [Google Scholar]
  53. DrwalM.N. BanerjeeP. DunkelM. WettigM.R. PreissnerR. ProTox: A web server for the in silico prediction of rodent oral toxicity.Nucleic Acids Res.201442W1W53W5810.1093/nar/gku401 24838562
     [Google Scholar]
  54. BanerjeeP. KemmlerE. DunkelM. PreissnerR. ProTox 3.0: A webserver for the prediction of toxicity of chemicals.Nucleic Acids Res.202452W1W513W52010.1093/nar/gkae303 38647086
     [Google Scholar]
  55. MerdekawatiF. In silico study of pyrazolylaminoquinazoline toxicity by lazar, protox, and admet predictor.J. Appl. Pharm. Sci.20188911912910.7324/JAPS.2018.8918
     [Google Scholar]
  56. MankadH. MonilM.A.H. RaoS. ColellaP. van StraalenB. FranchettiF. VetterJ.S. A Performance-Portable MultiGPU Implementation of 3D Euler Equations Using ProtoX and IRIS. Proc. SC Workshops 2024: Workshops of the International Conference for High Performance Computing, Networking, Storage and AnalysisDenver, COIEEE/ACM20241723173110.1109/SCW63240.2024.00215
     [Google Scholar]
  57. EnüstünB.V. ŞentürkH.S. YurdakulO. Capillary freezing and melting.J. Colloid Interface Sci.197865350951610.1016/0021‑9797(78)90103‑0
     [Google Scholar]
  58. RechnitzerL.A. TevebaughA.D. Apparatus to measure high temperature melting points by the capillary tube method.Rev. Sci. Instrum.196031221521610.1063/1.1716941
     [Google Scholar]
  59. SavvatimskiyA.I. Measurements of the melting point of graphite and the properties of liquid carbon (a review for 1963–2003).Carbon20054361115114210.1016/j.carbon.2004.12.027
     [Google Scholar]
  60. TiersG.V.D. Calibration of capillary melting-point apparatus to the international temperature scale of 1990 (ITS-90) by use of fluxed highly pure metals.Anal. Chim. Acta199023724124410.1016/S0003‑2670(00)83924‑0
     [Google Scholar]
  61. BunceS.C. Thermometer calibration for determination of capillary melting points.Anal. Chem.195325582582610.1021/ac60077a049
     [Google Scholar]
  62. KausarN. MukherjeeP. DasA.R. A practical carbocatalysis by graphene oxide nanosheets in aqueous medium towards the synthesis of diversified dibenzo[1,4]diazepine scaffold.RSC Adv.20166911910.1039/C6RA17520A
     [Google Scholar]
  63. RawatP. KhanM.F. KumarM. TamarkarA.K. SrivastavaA.K. AryaK.R. MauryaR. Constituents from fruits of Cupressus sempervirens.Fitoterapia201081316216610.1016/j.fitote.2009.08.014 19686818
     [Google Scholar]
  64. DuttaB. JanaR. BhanjaA.K. RayP.P. SinhaC. MirM.H. Supramolecular aggregate of Cadmium (II)-based one-dimensional coordination polymer for device fabrication and sensor application.Inorg. Chem.20195842686269410.1021/acs.inorgchem.8b03294 30698955
     [Google Scholar]
  65. SaxenaR. MeenaP.L. Flow injection online solid phase extraction system using Amberlite XAD-16 functionalized with 8-hydroxyquinoline for copper and zinc determination by flame atomic absorption spectrometry.RSC Adv.2014439202162022510.1039/C4RA01260G
     [Google Scholar]
  66. MondalS. MandalS.M. MondalT.K. SinhaC. Spectroscopic characterization, antimicrobial activity, DFT computation and docking studies of sulfonamide Schiff bases.J. Mol. Struct.2017112755756710.1016/j.molstruc.2016.08.011
     [Google Scholar]
  67. GanZ. HungI. WangX. PaulinoJ. WuG. LitvakI.M. Gor’kovP.L. BreyW.W. LendiP. SchianoJ.L. BirdM.D. DixonI.R. TothJ. BoebingerG.S. CrossT.A. NMR spectroscopy up to 35.2 T using a series-connected hybrid magnet.J. Magn. Reson.201728412513610.1016/j.jmr.2017.08.007 28890288
     [Google Scholar]
  68. AlleyK.A. ClarksonA.J. UeharaA. JohnsonJ.S. A site-specific synthetic route to substituted inda (box) ligands.Org. Lett.2023255191089113 38096808
     [Google Scholar]
  69. LøhreC. UnderhaugJ. BruslettoR. BarthT. A workup protocol combined with direct application of quantitative nuclear magnetic resonance spectroscopy of aqueous samples from large-scale steam explosion of biomass.ACS Omega20216106714672110.1021/acsomega.0c05642 33748585
     [Google Scholar]
  70. CallonM. MalärA.A. PfisterS. ŘímalV. WeberM.E. WiegandT. ZehnderJ. ChávezM. CadalbertR. DebR. DäppA. FogeronM.L. HunkelerA. LecoqL. TorosyanA. ZylaD. GlockshuberR. JonasS. NassalM. ErnstM. BöckmannA. MeierB.H. Biomolecular solid-state NMR spectroscopy at 1200 MHz: The gain in resolution.J. Biomol. NMR2021756-725527210.1007/s10858‑021‑00373‑x 34170475
     [Google Scholar]
  71. SchelbleS.M. CronkW.C. BucknerJ. Scaffolding NMR Teaching and Learning at an Undergraduate Program in a Virtual World. NMR Spectroscopy in the Undergraduate Curriculum, Volume 4: In-Person and Distance Learning ApproachesACS Symposium Series; American Chemical SocietyWashington, DC2021234010.1021/bk‑2021‑1376.ch003
     [Google Scholar]
  72. PykaA. Detection progress of selected drugs in TLC.BioMed Res. Int.20142014111910.1155/2014/732078 24551853
     [Google Scholar]
  73. ChengS.C. HuangM.Z. ShieaJ. Thin layer chromatography/mass spectrometry.J. Chromatogr. A20111218192700271110.1016/j.chroma.2011.01.077 21334632
     [Google Scholar]
  74. KalászH. BáthoriM. Pharmaceutical applications of TLC.LC GC Eur.2001145311321
     [Google Scholar]
  75. ArmstrongD.W. Pseudophase liquid chromatography: Applications to TLC.J. Liq. Chromatogr.19803689590010.1080/01483918008060200
     [Google Scholar]
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Synthesis, Computational Studies, and Biological Evaluation of Chalcones as Antifungal Agents
Anti Infect. Agents 24, E22113525369092 (2026); https://doi.org/10.2174/0122113525369092250309111235
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  • Article Type:     Research Article
Keyword(s): antifungal activity; biological evaluation; Chalcones; computational studies; molecular docking; physicochemical parameter

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