Protocols for Visualizing and Quantifying Receptors in Ocular Tissues Involved in Aqueous Humor Inflow and Outflow

Preview this chapter:

Protocols for Visualizing and Quantifying Receptors in Ocular Tissues Involved in Aqueous Humor Inflow and Outflow, Page 1 of 1

< Previous page | Next page > /docserver/preview/fulltext/9789815324334/chapter-6-1.gif

Glaucomatous optic neuropathy (GON) globally afflicts millions of patients and gradually but progressively robs them of their eyesight over decades of undetected and undiagnosed disease progression. Chronically raised intraocular pressure (IOP), also known as ocular hypertension (OHT), is most significantly associated with the development of GON and is the only modifiable and treatable biomarker for the most prevalent form of glaucoma, open-angle glaucoma (OAG). There continues to be a need to discover and introduce new drugs with good therapeutic indices in the clinical management of OAG. Potential targets are being discovered from genetic studies, but their localization and verification in the target tissues and cells in the anterior chamber (ANC) of the eye need to be determined before embarking on initiating drug discovery campaigns. Several techniques have been developed for target verification. This chapter deals with quantitative autoradiographic procedures to visualize receptor targets for drug candidates and existing marketed medicines treating OHT and OAG.