Protocol for Single-Cell RNA-Sequence Analysis of Genetic Models of Glaucoma
- Authors: Jack Hopkins1, Alex Leung2, Youichi Shinozaki3, Schuichi Koizumi4, Shin ichi Ohnuma5
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View Affiliations Hide Affiliations1 UCL Institute of Ophthalmology, University College London, London, United Kingdom 2 UCL Institute of Ophthalmology, University College London, London, United Kingdom 3 Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine & GLIA Center, University of Yamanashi, Yamanashi, Japan 4 Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine & GLIA Center, University of Yamanashi, Yamanashi, Japan 5 UCL Institute of Ophthalmology, University College London, London, United Kingdom
- Source: Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma - Ocular Hypertension , pp 522-531
- Publication Date: August 2025
- Language: English
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We used two genetically modified mouse models to elucidate the role of different cell types in the initiation and progression of glaucoma. Retinal ganglion cell death has been strongly associated with glaucoma development, and several key genes expressed in certain cell types have been identified. This includes the astrocyte-specific ABCA1 gene and the P2RY6 gene, which are found in non-pigmented epithelial cells of the ciliary body. This section describes the protocols for performing scRNA-sequencing to elucidate crucial mechanisms in cell populations and data analysis of sequencing data using the 10X Genomics platform and Seurat package in R.
Hardbound ISBN:
9789815324341
Ebook ISBN:
9789815324334
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