Protocol for Inducing Ocular Hypertension in Rats and Mice Using Circumlimbal Sutures
- Authors: Da Zhao1, Zheng He2, Pei Ying Lee3, Brianna Caroline Afiat4, Anna Van Koeverden5, Vickie Hoi Ying Wong6, Christine Tram Oanh Nguyen7, Bang Viet Bui8
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View Affiliations Hide Affiliations1 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 2 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 3 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 4 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 5 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 6 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 7 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia 8 Department of Optometry & Vision, 202 Berkeley St, Parkville, The University of Melbourne, Victoria 3010, Australia
- Source: Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma - Ocular Hypertension , pp 320-326
- Publication Date: August 2025
- Language: English
Protocol for Inducing Ocular Hypertension in Rats and Mice Using Circumlimbal Sutures, Page 1 of 1
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The circumlimbal suture technique for inducing experimental ocular hypertension (OHT) in rodents can be utilized to achieve chronic intraocular pressure (IOP) elevation, however, it is fully reversible. This protocol provides guidance for the application and adjustments of the suturing approach for both rats and mice. In brief, by placing the animals under general anesthesia, a "purse-string" suture is threaded through and anchored to the conjunctiva around the equator, behind and parallel to the eyeball limbus. Using this procedure, moderate levels of IOP increases can be achieved over a 12-week time period. This protocol can induce retinal dysfunctions, specifically both structural and functional, commensurate with retinal ganglion cell demise and optic nerve damage. Removal of the suture and restoration of IOP back to baseline levels afford functional recovery when the intervention is early. This circumlimbal suture procedure is a minimally invasive and cost-effective means to induce OHT that reliably results in RGC and optic nerve injury in rodents. The effects of potential IOPlowering and neuroprotectants can be assessed in this novel animal model of OHT.
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