Protocols for Isolation and Characterization of Human Corneal Epithelial and Conjunctival Epithelial Cells

Preview this chapter:

Protocols for Isolation and Characterization of Human Corneal Epithelial and Conjunctival Epithelial Cells, Page 1 of 1

< Previous page | Next page > /docserver/preview/fulltext/9789815324334/chapter-13-1.gif

The eye is a specialized organ composed of many types of tissues and cells. Since the ocular surface is the first point of contact with light entering the eye, environmental airborne materials, and topically applied medications, it is important to understand the features of these cells and their physiopathology. Furthermore, such characteristics and receptor/enzyme/transporter profiles of the cells can serve as targets for drug discovery and development to treat such eye disorders and/or to show the potential risks of ocular irritation and inflammation associated with certain medications. This is particularly important with respect to corneal and conjunctival epithelial cells and mast cells, which are involved in the disease mechanisms associated with dry eye syndrome, ocular allergies, and ocular surface pain. The isolated cells can also be used to study the mechanisms of actions of certain drugs such as antihistamines, mast cell stabilizers, and steroids. This chapter aims to provide protocols to isolate, propagate, and study cells obtained from human cadaveric donor tissues. Potential ways to immortalize human corneal epithelial cells will also be described.