Protein Purification
- Authors: Sinchan Hait1, Sai Joshi2, Ankita Nitin Padhye3
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View Affiliations Hide Affiliations1 Department of Chemical Engineering, BITS Pilani K.K. Birla Goa Campus, Goa-403726, India 2 CSIR-NCL, Dr. Homi Bhabha Road, Pune - 411 008, India 3 Food Engineering and Technology Department, Institute of Chemical Technology, Mumbai, Maharashtra, India
- Source: Practical Biochemistry , pp 128-152
- Publication Date: August 2024
- Language: English
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Protein purification is an essential step after protein isolation. Protein purification ensures the removal of all contaminants from the sample pool. Purification depends on the chemical and physical interactions of protein molecules and chemical moieties. These techniques are performed with tags and without tags. Tag purification involves ammonium sulfate precipitation and dialysis. Purification of proteins with tags utilizes affinity and size exclusion chromatography. There are various methods available for this, however, we will discuss some most common methods for protein purification.
Hardbound ISBN:
9789815165869
Ebook ISBN:
9789815165852
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