Quantitative Tests for Nucleic Acid Detection
- Authors: Mrittika Sarkar1, Sinchan Hait2, Sai Joshi3, Revati Supekar4
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View Affiliations Hide Affiliations1 Department of Biomedical Engineering, University of Michigan, Michigan, USA 2 Department of Chemical Engineering, BITS Pilani K.K. Birla Goa Campus, Goa-403726, India 3 CSIR-NCL, Dr. Homi Bhabha Road, Pune - 411 008, India 4 Biotechnology, Department of Management Studies, DY Patil University, Pune, India
- Source: Practical Biochemistry , pp 176-193
- Publication Date: August 2024
- Language: English
Quantitative Tests for Nucleic Acid Detection, Page 1 of 1
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Nucleic acid quantification is essential in experiments associated with genetic engineering. Over periods, several techniques have been developed to quantify nucleic acids. UV-Vis spectrophotometer is the simplest technique where it is associated with absorbance, which is proportional to the concentration of the sample. There are some colourimetric methods, such as the diphenylamine (DPA) method, and the orcinol method, where nucleic acid reacts with diphenylamine and forms a bright blue colour complex, and orcinol reacts and forms a green colour complex. Apart from that, some advanced techniques, such as fluorescence techniques, are used for quantification. In this chapter, each of these methods is explained in detail.
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