Qualitative Tests for Nucleic Acid Detection
- Authors: Anshika Sah1, Mrittika Sarkar2, Sinchan Hait3, Sai Joshi4, Ahiree Ghosal5
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View Affiliations Hide Affiliations1 Environmental Studies and Resource Management, TERI School of Advanced Studies, New Delhi 110 070, India 2 Department of Biomedical Engineering, University of Michigan, Michigan, USA 3 Department of Chemical Engineering, BITS-Pilani K.K. Birla Goa Campus, Goa-403726, India 4 CSIR-NCL, Dr. Homi Bhabha Road, Pune - 411 008, India 5 Zydus School for Excellence, Ahmedabad, Gujarat, India
- Source: Practical Biochemistry , pp 162-175
- Publication Date: August 2024
- Language: English
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Qualitative tests of nucleic acids are important to detect the presence or absence of nucleic acid in the given sample. It can be done by performing agarose gel electrophoresis. Nucleic acids are negatively charged and hence can be attracted towards a positive charge in an electric field. This phenomenon is used in agarose gel electrophoresis to detect nucleic acids. Based on the mass of nucleic acids, different bands are formed in the gel, which can be visualized using fluorescent dyes.
Hardbound ISBN:
9789815165869
Ebook ISBN:
9789815165852
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