RESULTS:

Previously we have reviewed in this journal (Arakawa T. Kita Y. Curr. Protein Pept. Sci. 15 608-620 2014) the interaction of arginine with proteins and various applications of this solvent additive in the area of protein formulations and downstream processes. In this special issue we expand the concept of protein-solvent interaction into the analysis of the effects of solvent additives on various column chromatography including mixed-mode chromatography. Earlier in our research we have studied the interactions of such a variety of solvent additives as sugars salts amino acids polymers and organic solvents with a variety of proteins which resulted in mechanistic understanding on their protein stabilization and precipitation effects the latter known as Hofmeister series. While such a study was then a pure academic research rapid development of genetic engineering technologies and resultant biotechnologies made it a valuable knowledge in fully utilizing solvent additives in manipulation of protein solution including column chromatography.