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Probiotics are live microorganisms. If administered in adequate amounts, they provide a health benefit to the host. They revolutionize health science and dominate the food industry today. Their mechanisms of action include competitive exclusion of pathogens, modulation of the immune system, strengthening of the intestinal barrier, and regulation of the gut-brain axis. These functions suggest their wide-ranging potential applications in a number of conditions, including gastrointestinal disorders, allergies, cancer, and cardiovascular diseases. The advent of functional foods has also changed the traditional paradigm of dietary elements as conveyor belts for disease prevention or health promotion. However, these wonder organisms are full of plausible obstacles to their practical application, such as susceptibility to the various stresses of gastrointestinal and industrial processing, which constrain their viability and effectiveness. Some of the recent advances like nanoencapsulation, genetic engineering, and new delivery systems have opened up new lines for tackling these challenges. These fundamentally include stability, bioavailability, and targeted delivery of probiotics. These aspects open really new horizons. This letter, therefore, stresses the need for multidisciplinary research to adequately optimize these technologies toward the safe and effective application possibilities of probiotics in the healthcare and food industries. Once these barriers are overcome, they are in a position not only to change human health but also to pave the way for innovative and sustainable products across a variety of industrial domains.