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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Calcineurin Hydrolysis of Para-Nitrophenyl Phosphorothioate
- Source: Protein and Peptide Letters, Volume 11, Issue 2, Apr 2004, p. 149 - 158
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- 01 Apr 2004
Abstract
para-Nitrophenyl phosphorothioate (pNPT) was hydrolyzed by calcineurin at initial rates slightly, but comparable to rates for para-nitrophenyl phosphate (pNPP). Kinetic characterization yielded higher estimates for both Km and Vmax compared to pNPP. Metal ion activation of phosphorothioate hydrolysis was more promiscuous. Unlike the hydrolysis of with pNPP, Ca2+, Mg2+, and Ba2+ activated calcineurin as well as Mn2+.
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