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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Urea-Induced Denaturation of β-Trypsin: An Evidence for a Molten Globule State
- Source: Protein and Peptide Letters, Volume 11, Issue 2, Apr 2004, p. 133 - 140
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- 01 Apr 2004
Abstract
The denaturation of β-trypsin induced by urea was investigated by fluorescence and circular dichroism. A transient denatured state was found at 2 M urea in both intrinsic fluorescence spectrum and bis-(8-anilino-1-naphtalene sulfonate) (bis-ANS) binding. In addition, the absence of tertiary contacts and presence of secondary structure for this state, are consistent with an intermediate equilibrium state having features of molten globule.
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