Chemical Synthesis of Ns-1 Region of Hepatitis C-Viral Polyprotein Fragments: A Comparison of Ps-Bdodma Resin and Merrifield Resin

Three peptide fragments selected from the NS-1 region of hepatitis C-viral polyprotein(Leu-Ile-Asn-Thr-Asn-Ala-Ser-Trp-His-Ala-Asn-Arg-Thr-Ala-Leu-Ser-Asn- Asp-Ser-Lys-Leu-Asn-Thr-Gly-Ala-NH2, Leu-lle-Asn-Thr-Asn-Ala-Ser-Trp-His-Ala-Asn-Arg-Thr-Ala-NH2 and Leu-Asn-Cys(Acm)-Asn-Asp-Ser-Leu-Asn-Thr-Ala-NH2) [1] have been synthesized on PS-BDODMA resin. The synthetic capability of the resin PS-BDODMA resin was compared with Merrifield resin. The peptides were synthesized by the stepwise fluoren-9-yl methoxycarbonyl (Fmoc) solid-phase method. The synthesized peptides were purified by HPLC and the identity of the peptides was established by mass spectrum and amino acid analysis. The synthesis of these peptides illustrates the application of the PS-BDODMA resin for the synthesis of long chain peptides in high yield and homogeneity compared to the Merrifield resin.