Synthesis and Biological Effects of an Anorectic Peptide Cart (Cocaine- And Amphetamine-Regulated Trascript) (Human55-102) Analogue

[Phe(4F)97]CART55-102 was synthesized by a solid-phase method. Hydrogen fluoride was used to deprotect and cleave the peptide from the resin except for Acm groups and a Met(0) residue. The product was treated with dithiothreitol to remove a sulfoxide group of Met(0) residue and then treated with Hg(OAc)2 in 50percent aqueous AcOH to remove Acm groups. The reduced peptide was subjected to oxidative folding to form disulfide bridges. The crude product was purified by PR-HPLC. The synthetic [Phe(4F)97]CART55-102 and CART55-102 were tested for comparative effect on inhibitory food intake in rats. This peptide exhibited far stronger inhibitory effect than that of the synthetic CART55-102