Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Uraria picta Leaves Extract

Background: The rapid synthesis and antimicrobial activity of silver nanoparticles (AgNPs) synthesized using Uraria picta extract were investigated in this paper, and AgNPs were examined for antimicrobial activity against a variety of pathogenic organisms, including bacteria and fungi. Objective: The main objective was to synthesize AgNPs from Uraria picta leaves extract by the technique of green synthesis for antimicrobial evaluation against bacteria and fungi using MIC studies. Methods: The AgNPs were formed by treating an aqueous extract of Uraria picta leaves with silver nitrate (1 mM) solution, and then nanoparticles were synthesized for various studies. Results: The Uraria picta leaves extract can be used for the green synthesis of AgNPs effectively. The absorption band at 425 nm in the UV-Vis spectrum confirmed the synthesis of AgNPs. According to MIC tests, silver nanoparticles exhibited antimicrobial and antifungal properties. This work will provide a better understanding of the development of new antimicrobial and antifungal activities. Conclusion: We utilized Uraria picta aqueous leaves extract to develop a fast, cost-effective, ecofriendly, and simple method for the synthesis of AgNPs, and the nanoparticles synthesized by this plant for the first time demonstrated antimicrobial activity. AgNPs were observed to be spherical and oval in shape, with average particle sizes ranging from 12.54 to 25.58 nm. The antimicrobial activity of AgNPs was confirmed against typhi, Escherichia coli, Bacillus subtilis, and Staphylococcus aureus by a significant zone of inhibition.