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- ISSN: 1872-3128
- E-ISSN: 1874-0758
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Impact of Time-Dependent Inactivation on the Estimation of Enzyme Kinetic Parameters for Midazolam
- Source: Drug Metabolism Letters, Volume 3, Issue 1, Jan 2009, p. 45 - 53
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- 01 Jan 2009
Abstract
Enzyme kinetic parameters for midazolam were time-dependent in human liver microsomes, under initial velocity conditions. Vmax and Km decreased up to 3.7 and 3.1-fold, respectively, for 10 min compared to 1 min incubations. Mathematical models describing the relationship between inactivation and the time-dependency of enzyme kinetic parameter estimates were derived and discussed.
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