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- ISSN: 1872-3128
- E-ISSN: 1874-0758
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Comprehensive Identification of Cytochrome P450 Isoforms from Solubility-Based Fractionated Rat Liver Microsomes
- Source: Drug Metabolism Letters, Volume 1, Issue 4, Dec 2007, p. 281 - 286
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- 01 Dec 2007
Abstract
Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.
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