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2000
Volume 20, Issue 11
  • ISSN: 1574-888X
  • E-ISSN: 2212-3946

Abstract

Background

Burn injuries pose a significant health challenge, leading to intense physiological stress compared to other types of trauma. Myrtle has been traditionally used for treating various skin ailments, while mesenchymal stem cells (MSCs) have introduced innovative approaches for burn treatment. This study aimed to evaluate the impact of myrtle compared to MSCs on the healing of deep second-degree burns.

Methods

Fifty adult male albino rats were randomly divided into five groups: Group A served as the control, Group B received an excision burn without treatment, Group C was treated with topical myrtle paste, Group D received an intradermal injection of mesenchymal stem cells (MSCs), and Group E received both topical myrtle paste and intradermal MSC injection. Burn healing was assessed based on visible characteristics over 21 days. At the end of the treatment, skin samples were collected for biochemical analysis, histological examination using hematoxylin and eosin (H&E) staining, and VEGF concentration measurement ELISA. Additionally, PCR analysis was conducted to assess the expression levels of COL1a1, COL3a1, TNF-α, and IL-6, providing insights into collagen production and inflammatory response.

Results

Gross evaluation and histopathological analysis indicated that Groups D and E exhibited complete skin regeneration compared to the burn group. VEGF analysis demonstrated enhanced angiogenesis in the treated groups. PCR analysis revealed upregulation of COL1a1 and COL3a1, along with downregulation of TNF-α and IL-6, suggesting reduced inflammation and improved skin healing.

Conclusion

The study demonstrated that both MSCs and myrtle contributed to significant burn healing. The combination of myrtle and MSCs (Group E) exhibited the most effective skin regeneration, likely due to enhanced collagen production, reduced inflammation, and improved angiogenesis. These findings suggest that combining traditional herbal treatments with stem cell therapy may offer a promising strategy for burn management.

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2026-02-05

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References

  1. SokolovVEe. Mammal skin.University of California Press20233770410.2307/jj.5232967
     [Google Scholar]
  2. MonseauA.J. ReedZ.M. LangleyK.J. OnksC. Sunburn, thermal, and chemical injuries to the skin.Prim. Care201542459160510.1016/j.pop.2015.07.003 26612374
     [Google Scholar]
  3. PeckM.D. ToppiJ.T. Epidemiology and prevention of burns throughout the world.Springer202010.1007/978‑3‑030‑18940‑2_2
     [Google Scholar]
  4. Markiewicz-GospodarekA. KoziołM. TobiaszM. BajJ. Radzikowska-BüchnerE. PrzekoraA. Burn wound healing: Clinical complications, medical care, treatment, and dressing types: the current state of knowledge for clinical practice.Int. J. Environ. Res. Public Health2022193133810.3390/ijerph19031338 35162360
     [Google Scholar]
  5. SharifiE. ChehelgerdiM. Fatahian-KelishadrokhiA. Yazdani-NafchiF. Ashrafi-DehkordiK. Comparison of therapeutic effects of encapsulated Mesenchymal stem cells in Aloe vera gel and Chitosan-based gel in healing of grade-II burn injuries.Regen. Ther.202118303710.1016/j.reth.2021.02.007 33816723
     [Google Scholar]
  6. ShengG. The developmental basis of mesenchymal stem/stromal cells (MSCs).BMC Dev. Biol.20151514410.1186/s12861‑015‑0094‑5 26589542
     [Google Scholar]
  7. Guillamat-PratsR. The role of MSC in wound healing, scarring and regeneration.Cells2021107172910.3390/cells10071729 34359898
     [Google Scholar]
  8. LopesB. The application of mesenchymal stem cells on wound repair and regeneration.Appl. Sci.2021117300010.3390/app11073000
     [Google Scholar]
  9. Nancarrow-LeiR. MafiP. MafiR. KhanW. A systemic review of adult mesenchymal stem cell sources and their multilineage differentiation potential relevant to musculoskeletal tissue repair and regeneration.Curr. Stem Cell Res. Ther.201712860161010.2174/1574888X12666170608124303 28595566
     [Google Scholar]
  10. SinghN. GargM. ChopraR.J.I. An overview of herbal formulations: From processing to pharmacovigilance.IJPSR202314256257810.13040/IJPSR.0975‑8232.14(2).562‑78
     [Google Scholar]
  11. GiampieriF. CianciosiD. Forbes‐HernándezT.Y.J.F.F. Myrtle (Myrtus communis L.) berries, seeds, leaves, and essential oils: New undiscovered sources of natural compounds with promising health benefits.Food Frontiers20201327629510.1002/fft2.37
     [Google Scholar]
  12. ElbaghdadyH.A.M. AlwailiM.A. EL-Demerdash RS. Amelioration of cadmium-induced testes’ damage in rats by the bone marrow mesenchymal stem cells.Ecotoxicol. Environ. Saf.201814876376910.1016/j.ecoenv.2017.10.016 29182986
     [Google Scholar]
  13. ImbarakN. Abdel-AzizH.I. FarghalyL.M. HosnyS. Effect of mesenchymal stem cells versus aloe vera on healing of deep second-degree burn.Stem Cell Investig.202181210.21037/sci‑2020‑030 34268441
     [Google Scholar]
  14. OryanA. AlemzadehE. MohammadiA.A. MoshiriA. Healing potential of injectable Aloe vera hydrogel loaded by adipose-derived stem cell in skin tissue-engineering in a rat burn wound model.Cell Tissue Res.2019377221522710.1007/s00441‑019‑03015‑9 30923912
     [Google Scholar]
  15. MaqboolT. AwanS.J. MalikS. HadiF. ShehzadiS. TariqK. In-vitro anti-proliferative, apoptotic and antioxidative activities of medicinal herb Kalonji (Nigella sativa).Curr. Pharm. Biotechnol.201920151288130810.2174/1389201020666190821144633 31433749
     [Google Scholar]
  16. LaneC.A. WaitesK.G.J.P.M. ClinicsR. General skin issues in the adult rehabilitation population.Phys. Med. Rehabil. Clin. N. Am.202233474575810.1016/j.pmr.2022.06.001 36243467
     [Google Scholar]
  17. JeschkeM.G. van BaarM.E. ChoudhryM.A. ChungK.K. GibranN.S. LogsettyS. Burn injury.Nat. Rev. Dis. Primers2020611110.1038/s41572‑020‑0145‑5 32054846
     [Google Scholar]
  18. HuangY.Z. GouM. DaL.C. ZhangW.Q. XieH.Q. Mesenchymal stem cells for chronic wound healing: Current status of preclinical and clinical studies.Tissue Eng. Part B Rev.202026655557010.1089/ten.teb.2019.0351 32242479
     [Google Scholar]
  19. KazemnejadS. Characteristics of Mesenchymal Stem Cells Derived from Amniotic Membrane: A Potential Candidate for Stem Cell-Based Therapy.Perinatal Tissue-Derived Stem Cells Stem Cell Biology and Regenerative Medicine.ChamHumana Press2016137169
     [Google Scholar]
  20. RasulovM.F. Vasil’chenkovA.V. OnishchenkoN.A. First experience in the use of bone marrow mesenchymal stem cells for the treatment of a patient with deep skin burns.Bull. Exp. Biol. Med.2005139114114410.1007/s10517‑005‑0232‑3 16142297
     [Google Scholar]
  21. RasulovM.F. VasilenkoV.T. ZaidenovV.A. OnishchenkoN.A. Cell transplantation inhibits inflammatory reaction and stimulates repair processes in burn wound.Bull. Exp. Biol. Med.2006142111211510.1007/s10517‑006‑0306‑x 17369918
     [Google Scholar]
  22. VelnarT. BaileyT. SmrkoljV. The wound healing process: An overview of the cellular and molecular mechanisms.J. Int. Med. Res.20093751528154210.1177/147323000903700531 19930861
     [Google Scholar]
  23. MoaliC. HulmesD.J.S. Extracellular and cell surface proteases in wound healing: New players are still emerging.Eur. J. Dermatol.200919655256410.1684/ejd.2009.0770 19625234
     [Google Scholar]
  24. Zaja-MilatovicS RichmondA. CXC chemokines and their receptors: A case for a significant biological role in cutaneous wound healing.200823111399407 18785122
     [Google Scholar]
  25. BehmB. BabilasP. LandthalerM. SchremlS. Cytokines, chemokines and growth factors in wound healing.J. Eur. Acad. Dermatol. Venereol.201226781282010.1111/j.1468‑3083.2011.04415.x 22211801
     [Google Scholar]
  26. SierawskaO. MałkowskaP. TaskinC. Innate immune system response to burn damage—focus on cytokine alteration.Int. J. Mol. Sci.202223271610.3390/ijms23020716 35054900
     [Google Scholar]
  27. BarrientosS. StojadinovicO. GolinkoM.S. BremH. Tomic-CanicM. PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing.Wound Repair Regen.200816558560110.1111/j.1524‑475X.2008.00410.x 19128254
     [Google Scholar]
  28. HamidA.A. Soliman MJJoH. Effect of topical aloe vera on the process of healing of full-thickness skin burn: A histological and immunohistochemical study.J Histol Histopathol201521310.7243/2055‑091X‑2‑3
     [Google Scholar]
  29. Ashkani-EsfahaniS. ImaniehM.H. KhoshneviszadehM. The healing effect of arnebia euchroma in second degree burn wounds in rat as an animal model.Iran. Red Crescent Med. J.20121427074 22737558
     [Google Scholar]
  30. HäkkinenL. LarjavaH. KoivistoL.J.E.T. Granulation tissue formation and remodeling.Endod. Topics20112419412910.1111/etp.12008
     [Google Scholar]
  31. AleemM. Anis MJJoP. Therapeutic potential of Habb-ul-Aas (Myrtus communis Linn.) with unani perspective and modern pharmacology: A review.J. Pharmacogn. Phytochem.202110191092310.22271/phyto.2021.v10.i1m.13452
     [Google Scholar]
  32. GiacomelliC. NataliL. NisiM. Negative effects of a high tumour necrosis factor-α concentration on human gingival mesenchymal stem cell trophism: The use of natural compounds as modulatory agents.Stem Cell Res. Ther.20189113510.1186/s13287‑018‑0880‑7 29751776
     [Google Scholar]
/content/journals/cscr/10.2174/011574888X351632250529121312
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Regenerative Therapy for Deep Burn Injury using Mesenchymal Stem Cells and Myrtle (Myrtus Communis) in a Rat Model
Curr Stem Cell Res Ther 20, 1214 (2025); https://doi.org/10.2174/011574888X351632250529121312
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  • Article Type:     Research Article
Keyword(s): IL-6; MSCs; Myrtus communis; synergistic effect; VEGF; wound healing

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