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2000
Volume 18, Issue 5
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Objective: Linezolid is a significant antibiotic used against severe infections initiated by multi-resistant bacterial pathogens. Aims: The aim of this study is to develop and validate a simple, selective and accurate highperformance liquid chromatographic HPLC method for the analysis of linezolid LZD. Methods: Linezolid extraction from plasma is obtained using methanol. Chromatographic separation is achieved isocratically on a C18 column [Zorbax C18, 5 μm particle size, 150 mm* 4.6 mm] making use of a mobile phase of acetonitrile / 0.05 M phosphate buffer, pH = 4.5 (30: 70 v/v) at a flow rate of 1.2 mL/min with photodiode array detector DAD, at a wavelength of 256 nm. Results: The retention time of linezolid was 2.5 min. The analytical method was linear (r2 > 0.998) over the calibration range of 0.30 to 50.0 μg/mL. The extraction recoveries of linezolid range from 71.03 to 91.93 %. The limit of quantification and the limit of detection were 0.112 μg and 0.037 μg, respectively. The RSDs for intraday and interday assays were < 7.77 and 4.32 %, respectively. The intraday and interday accuracies were in the range 80.6-112 % and 77.44- 104.85 %, respectively. Conclusion: The applied method is precise, accurate and appropriate for pharmacokinetic studies and therapeutic drug monitoring of linezolid in routine clinical practice.

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/content/journals/cpa/10.2174/1573412917666210823092454
2022-06-01
2024-11-14
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  • Article Type:
    Research Article
Keyword(s): biomedical analysis; human plasma; Linezolid; method development; RP-HPLC; validation
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