Simultaneous Determination of Five Irdoid Glycosides and Three Flavonoid Glycosides in Hedyotis Diffusa Wild by UPLC-UV with Ultrasound-Assisted Extraction

Background: Hedyotis diffusa is one of the most extensively used Chinese herbs for the treatment of varieties of cancers in the folk of China. The ingredients of the preparation are still not very clear and the quality control method of the herb medicine requires further improvement. Introduction: In this paper, the quantitative methods for the quality control of H. diffusa were originally developed by using UPLC-UV. Methods: Using the five irdoid glycosides and three flavonoid glycosides previously obtained from H. diffusa as index components, referring to 10-Acetyl Scandoside Methyl Ester (ASME), Scandoside (SD), Deacetyl Aspemloside (DA), Asperulosidic Acid (AA), Asperuloside (AD), Qurecetin-3-OSophoroside (QS), Quercetin-3-O-[2-(6-O-E-Sinapoyl-β-D-Glucopyranosyl]-β-D-Glucopyranosyl (QESGG), and Quercetin-3-O-[2-O-(6-O-E-Feruloyl)-β-D-Glucopyranosyl]-β-Glucopyanoside (QEFGG), the extraction conditions, including solvents, durations, and cycles were optimized firstly. Subsequently, a new UPLC-UV method was originally established to simultaneously determine the eight ingredients in the Chinese herbal medicine. Results: The validated method exhibited good linearity (R2 > 0.999), repeatability (RSD < 3.01%), intra- and inter-day precision (RSD <6.19%) and recoveries (96.31% to 104.22%) for the simultaneous determination of the eight ingredients studied and had been used for the quality analysis of 6 batches of H. diffusa sameples. Conclusion: The established UPLC-UV method could serve as a rapid and effective tool for quality evaluation of the herbal medicine, H. diffusa.