Combinatorial Application of Multiple High-throughput Biotechnologies for the Study of Autoimmune Diseases

Currently more and more high-throughput biotechnologies have been armed by immunologists to explore the pathological mechanism of different autoimmune diseases. The new wave of approaches for the integrated understanding of human immune system based on high-throughput methods, corresponding mathematical and computational tools are increasing. This paper described the combination tendency of multiple methods to investigate the whole immune process at different levels including transcription, translation and post-translation modification, using microarray, Luminex and CyTOF as an example. When proper high-throughput technologies were used in combination, there will be more comprehensive recognition on immune system. The application of such techniques will promote deeper and more integrated study in the immunization field, and become a promising study strategy in this field.