Application and Development of Targeted Fishing Technology in Natural Product Screening - A Simple Minireview

Background: The screening of active ingredients in traditional Chinese medicine is an important task in the modernization of traditional Chinese medicine, and the commonly used analytical means in the past were mainly to screen the extracts of traditional Chinese medicine through pharmacological experiments, but the method has major defects. The target fishing strategy provides a new idea for the screening of active ingredients, and it has rapidly become a hot research direction, but there are many methods that need to be summarized and aggregated. Objective: It aims to provide readers with an understanding of the achievements, developments, and dilemmas of target fishing techniques over the past few years and to provide new ideas for subsequent research. Methods: Research articles in recent years using target fishing as an entry point are used as a basis to summarize the types of literature based on their principles and characteristics and to discuss the advantages and disadvantages of each method. Conclusion: This paper summarizes the classification and development of fishing techniques such as ultrafiltration, equilibrium dialysis, cell membrane chromatography, and immobilization of target molecules and target fishing and describes the principles and characteristics of these methods. The applications of these methods in the active ingredients of traditional Chinese medicine are summarized, and the problems and solutions of these methods are discussed.