Genetic Diversity of Anemone shikokiana (Makino) Makino Analyzed Using the Proteomics-Based Approach

In this study, we used a proteomics approach to analyze genetic diversity of a rare plant Anemone shikokiana (Makino) Makino, and further analyzed the influences of habitats and geographic distribution on it. Proteins were separated by two-dimensional electrophoresis (2-DE), visualized by Coomassie Brilliant Blue (CBB) staining, analyzed by ImageMaster software. A total of 1294 protein spots were detected in four gels from four populations across an isoelectric focusing range of 4-7, of which 1143(88.3%) were polymorphic among populations. By comparing analysis we found both of the habitats and geographic distribution influenced the biochemical changes. But the influence of geographic distribution on A. shikokiana was more important than that of habitat. 31 obviously different spots were found among four populations and identified by ESI-LC/MS/MS and homology searched in available database. The understanding of these proteins will increase our knowledge about conservation biology and improve our ability to conserve A. Shikokiana. Therefore proteomics is a powerful approach to analyze genetic diversity and influence the mechanism of habitats on the plants.