Mitochondrial Genomics and Proteomics

Factors involved in mitochondrial biogenesis and function have been studied classically via mutagenesis screens and subsequent genetic and biochemical analyses. The recent advent of high-throughput technologies has provided a wealth of information regarding mitochondrial function, morphology, gene regulation, protein complexes, and disease in a fraction of the time. This review will describe past and present genomic and proteomic methods used to study mitochondria both in yeast and mammalian cells, their advantages and limitations, and the current knowledge of the number of genes and proteins that are required for proper functioning of the organelle.