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Abstract

Introduction

Diabetes mellitus is a rapidly escalating global health concern, and numerous ethnobotanical remedies are under investigation for their antidiabetic properties. has long been used in traditional medicine by indigenous populations for glycemic control. This study aimed to characterize the phytochemical profile of and evaluate its α-glucosidase inhibition, antioxidant and antimicrobial activities.

Methods

Standard qualitative assays were used to identify secondary metabolites. Total phenolic content (TPC) was quantified Folin–Ciocalteu reagent (expressed as mg gallic acid equivalents [GAE]/g), and total flavonoid content (TFC) aluminum chloride colorimetric method (mg quercetin equivalents [QE]/g). Antioxidant potential was determined using the DPPH radical scavenging assay. α-Glucosidase inhibition was measured spectrophotometrically using p-nitrophenyl-α-D-glucopyranoside as a substrate. Antimicrobial efficacy against was tested using the agar well diffusion technique.

Results

Phytochemical screening confirmed the presence of alkaloids, flavonoids, glycosides, terpenoids, saponins, phenols, tannins, steroids, and quinones. TPC and TFC values were 181.41 ± 1.57 mg GAE/g and 12.08 ± 0.11 mg QE/g, respectively. The methanolic extract demonstrated considerable antioxidant activity (DPPH IC = 111.43 ± 1.13 µg/mL).

Discussion

Both crude and ethyl acetate extracts exhibited significant α-glucosidase inhibition (comparable or superior to control). Notable antibacterial activity was observed against , with a 9 mm inhibition zone.

Conclusion

The presence of diverse bioactive phytochemicals in supports its traditional use in diabetes management. Its potent α-glucosidase inhibition suggests a mechanism for attenuating postprandial hyperglycemia, while antioxidant and antimicrobial activities substantiate additional therapeutic roles. These findings warrant further studies and mechanistic exploration to validate its potential as a source of enzyme inhibitors and therapeutic agents for metabolic disorders.

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2025-08-18
2025-11-23

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Exploring α-Glucosidase Inhibitory, Antioxidant, and Antimicrobial Properties of Tinospora sinensis (Lour.) Merr.
Bentham Science Publishers ; https://doi.org/10.2174/0115734080391554250807044513
/content/journals/cei/10.2174/0115734080391554250807044513
/content/journals/cei/10.2174/0115734080391554250807044513
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  • Article Type:     Research Article
Keywords: anti-microbial ; Gurjo ; Antioxidant ; Tinospora sinensis ; Phytochemical screening ; α-glucosidase ; diabetes

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