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image of Investigations into the Biochemical Properties of Penicillin G Amidase in Collagen Hydrogel and Collagen Hydrogel + Gelatin Combination Bio-composite Beads as Carriers

Abstract

Introduction

Penicillin G amidase is an industrially significant enzyme widely employed in the production of semi-synthetic β-lactam antibiotics through the hydrolysis of Penicillin G to 6-aminopenicillanic acid. Owing to its commercial importance, extensive research has focused on improving the operational stability, reusability, and catalytic efficiency of PGA through various immobilization strategies.

Methods

Optimization of multiple parameters for free and immobilized Penicillin G Acylase (PGA) is critical for improving the enzyme's catalytic effectiveness, stability, and reusability in industrial and medicinal applications. This procedure entails methodically altering and analyzing variables such as substrate concentration, mechanical stability, cycle number, and storage conditions, and their effects on operational stability, pH, and temperature. PGA was optimized by entrapment on collagen hydrogel beads, resulting in collagen hydrogel + gelatin hybrid gel beads.

Results

Immobilized PGA in Collagen Hydrogel + gelatin hybrid beads showed superior thermal stability, reusability, and storage stability as compared to gelatin-immobilized PGA. The entrapment of PGA onto Collagen Hydrogel + gelatin hybrid beads revealed several advantages and could be used in the production of 6-aminopenicillanic acid (6APA).

Discussion

The study investigated the biochemical behavior of Penicillin G amidase (PGA) immobilized on collagen hydrogel and a collagen–gelatin bio-composite. Relative analysis focused on enzyme activity, stability, and mechanical strength, revealing insights into their appropriateness as immobilization matrices for enhanced PGA performance in industrial biocatalysis applications.

Conclusion

Hydrogel + gelatin hybrid beads are more beneficial in industrial applications due to their greater stability and usability. PGA entrapment onto Hydrogel + gelatin hybrid beads has shown numerous advantages and may be useful in the manufacture of 6APA (6-aminopenicillanic acid).

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2025-09-24
2025-12-25

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References

  1. Yang Y. Biedendieck R. Wang W. High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium. Microb. Cell Fact. 2006 5 1 36 10.1186/1475‑2859‑5‑36 17132166
    [Google Scholar]
  2. Sitnikov N.S. Li Y. Zhang D. Yard B. Schmalz H.G. Design, synthesis, and functional evaluation of CO-releasing molecules triggered by Penicillin G amidase as a model protease. Angew. Chem. Int. Ed. 2015 54 42 12314 12318 10.1002/anie.201502445 26037072
    [Google Scholar]
  3. Basso A. Cantone S. Linda P. Ebert C. Stability and activity of immobilised penicillin G amidase in ionic liquids at controlled aw. Green Chem. 2005 7 9 671 676 10.1039/b506230f
    [Google Scholar]
  4. Margolin A. Svedas V. Berezin I. Substrate specificity of penicillin amidase from E. coli. Biochim. Biophys. Acta Enzymol. 1980 616 2 283 289 10.1016/0005‑2744(80)90145‑X
    [Google Scholar]
  5. Guisan J.M. Immobilization of enzymes and cells. 2nd ed New Jersey, USA Humana Press Inc. 2006 10.1007/978‑1‑59745‑053‑9
    [Google Scholar]
  6. Matsumoto K. Production of 6-APA, 7-ACA, and 7-ADCA by immobilized penicillin and cephalosporin amidases. Bioprocess Technol. 1993 16 67 88 7764037
    [Google Scholar]
  7. Sheldon R.A. Enzyme immobilization: The quest for optimum performance. Adv. Synth. Catal. 2007 349 8-9 1289 1307 10.1002/adsc.200700082
    [Google Scholar]
  8. Harguindeguy M. Antonelli C. Belleville M.P. Sanchez-Marcano J. Pochat-Bohatier C. Gelatin supports with immobilized laccase as sustainable biocatalysts for water treatment. J. Appl. Polym. Sci. 2021 138 2 49669 10.1002/app.49669
    [Google Scholar]
  9. Zhou Z. Li G. Li Y. Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate–chitosan beads. Int. J. Biol. Macromol. 2010 47 1 21 26 10.1016/j.ijbiomac.2010.04.001 20398691
    [Google Scholar]
  10. Huang T-C. Chen D-H. Wang S-S. Kinetic studies of penicillin g hydrolysis with immobilized penicillin acylase in an electrodialyzer. Chem. Eng. Commun. 1996 147 1 99 117 10.1080/00986449608936498
    [Google Scholar]
  11. Lee S.B. Ryu D.D.Y. Reaction kinetics and mechanisms of penicillin amidase: A comparative study by computer simulation. Enzyme Microb. Technol. 1982 4 1 35 38 10.1016/0141‑0229(82)90008‑4
    [Google Scholar]
  12. Alfonso C. Cribeiro L. Reyes F. Penicillin amidohydrolases in fungal autolysis. Microbiol. Immunol. 1989 33 1 69 74 10.1111/j.1348‑0421.1989.tb01498.x 2499747
    [Google Scholar]
  13. Kumar R. Suresh K.A. Shankar S.H. Kinetics, and reaction engineering of penicillin G hydrolysis. J. Chem. Technol. Biotechnol. 1996 66 3 243 250 10.1002/(SICI)1097‑4660(199607)66:3<243::AID‑JCTB488>3.0.CO;2‑O
    [Google Scholar]
  14. Lowry O. Rosebrough N. Farr A.L. Randall R. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951 193 1 265 275 10.1016/S0021‑9258(19)52451‑6 14907713
    [Google Scholar]
  15. Nupur N. Ashish E.Y. Debnath M. Preparation and biochemical property of penicillin g amidase-loaded alginate and alginate/chitosan hydrogel beads. Recent Pat. Biotechnol. 2016 10 1 121 132 10.2174/1872208310666160805112515 27494735
    [Google Scholar]
  16. Zuza M. Siler-Marinkovic S. Knezevic Z. Immobilization of penicillin acylase from Escherichia coli on commercial sepabeads EC-EP carrier. Acta Period. Technol. 2007 38 38 173 182 10.2298/APT0738173Z
    [Google Scholar]
  17. Andreazza R. Morales A. Pieniz S. Labidi J. Gelatin-based hydrogels: Potential biomaterials for remediation. Polymers 2023 15 4 1026 10.3390/polym15041026 36850309
    [Google Scholar]
  18. Xiao Q.G. Tao X. Zhang J-P. Chen J-F. Hollow silica nanotubes for immobilization of penicillin G acylase enzyme. J. Mol. Catal., B Enzym. 2006 42 1-2 14 19 10.1016/j.molcatb.2006.05.011
    [Google Scholar]
  19. Luo X. Zhang L. Immobilization of penicillin G acylase in epoxy-activated magnetic cellulose microspheres for improvement of biocatalytic stability and activities. Biomacromolecules 2010 11 11 2896 2903 10.1021/bm100642y 20919701
    [Google Scholar]
  20. eY. ChangZ. LuJ. Enzymatically mediated Gleditsia sinensis galactomannan based hydrogel inspired by wound healing process. Int J Biol Macromol 2023 230123152 10.1016/j.ijbiomac.2023.123152 36610566
    [Google Scholar]
  21. Elnashar M.M.M. Grafted carrageenan gel disks and beads with polyethyleneimine and glutaraldehyde for covalent immobilization of penicillin G acylase. J Colloid Sci Biotechnol 2013 2 1 7 10.1166/jcsb.2013.1029
    [Google Scholar]
  22. Bernardino S. Estrela N. Ochoa-Mendes V. Fernandes P. Fonseca L.P. Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties. J. Sol-Gel Sci. Technol. 2011 58 2 545 556 10.1007/s10971‑011‑2426‑7
    [Google Scholar]
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Investigations into the Biochemical Properties of Penicillin G Amidase in Collagen Hydrogel and Collagen Hydrogel + Gelatin Combination Bio-composite Beads as Carriers
Bentham Science Publishers ; https://doi.org/10.2174/0115734080374723250908050654
/content/journals/cei/10.2174/0115734080374723250908050654
/content/journals/cei/10.2174/0115734080374723250908050654
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  • Article Type:     Research Article
Keywords: carrier matrix optimization ; biochemical characterization ; Penicillin G amidase (PGA) ; Gelatin–collagen bio-composite beads ; industrial bio-catalysis applications ; Gelatin-collagen bio-composites ; enzyme immobilization

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