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image of Neuroprotective Potential of Oroxylum indicum Seeds in Antioxidant Activity, Enzyme Inhibition and Cognitive Improvement for Alzheimer's Disease

Abstract

Introduction

Alzheimer’s disease (AD) is a debilitating neurodegenerative disorder marked by progressive memory loss and cognitive decline. Oxidative stress and cholinergic dysfunction are central to its pathology. Natural products with antioxidant and cholinesterase-inhibitory activities are gaining attention as potential therapeutic agents. This study explores the neuroprotective potential of seed extract.

Methods

Methanolic extraction of seeds was followed by phytochemical screening. Bioactive compounds were isolated via column chromatography and characterized using FTIR, NMR, and mass spectrometry. Antioxidant potential was assessed through DPPH, nitric oxide (NO), and hydroxyl radical scavenging assays. acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition assays were conducted, and molecular docking was performed to evaluate binding affinities.

Results

Baicalein and chrysin were identified as major constituents. Both compounds showed potent antioxidant activity: baicalein (IC = 49.79 ± 0.033 µg/mL for DPPH, 30.48 ± 0.092 µg/mL for NO, and 48.56 ± 0.10 µg/mL for •OH) and chrysin (IC = 66.24 ± 0.032, 38.73 ± 0.030, and 52.18 ± 0.12 µg/mL, respectively). They also exhibited strong enzyme inhibition: baicalein (AChE: 40.73 µg/mL, BChE: 35.76 µg/mL) and chrysin (AChE: 52.74 µg/mL, BChE: 31.56 µg/mL). Docking studies supported these findings, showing strong binding affinities to the active sites of AChE and BChE.

Discussion

The strong free radical scavenging and cholinesterase-inhibitory effects of baicalein and chrysin highlight their potential to mitigate oxidative stress and cholinergic dysfunction in AD.

Conclusion

seed-derived baicalein and chrysin exhibit promising neuroprotective properties and represent potential candidates for the development of AD therapeutics.

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2025-06-25
2025-08-19

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References

  1. Hari Babu T. Manjulatha K. Suresh Kumar G. Gastroprotective flavonoid constituents from Oroxylum indicum Vent. Bioorg. Med. Chem. Lett. 2010 20 1 117 120 10.1016/j.bmcl.2009.11.024 19948405
    [Google Scholar]
  2. Mat Ali R. Houghton P.J. Raman A. Hoult J.R.S. Antimicrobial and antiinflammatory activities of extracts and constituents of Oroxylum indicum (L.) Vent. Phytomedicine 1998 5 5 375 381 10.1016/S0944‑7113(98)80020‑2 23195987
    [Google Scholar]
  3. Majumdar S. Mohanta B.C. Chowdhury D.R. Banik R. Dinda B. Basak A. Proprotein convertase inhibitory activities of flavonoids isolated from Oroxylum indicum. Curr. Med. Chem. 2010 17 19 2049 2058 10.2174/092986710791233643 20423311
    [Google Scholar]
  4. Halagali P. Nayak D. Tippavajhala V.K. Rathnanand M. Biswas D. Sharma H. Chapter 16 Navigating the nanoscopic frontier: Ethical dimensions in developing nanocarriers for neurodegenerative diseases In: Koduru TS, Osmani RAM, Singh E, Dutta SBTTNR, Eds.The Neurodegeneration Revolution: Emerging Therapies and Sustainable Solutions Koduru T.S. Osmani R.A.M. Singh E. Dutta S.B.T.T.N.R. United States Academic Press 2025 399 420 10.1016/B978‑0‑443‑28822‑7.00011‑8
    [Google Scholar]
  5. Halagali P. Nayak D. Rathnanand M. Tippavajhala V.K. Sharma H. Biswas D. Chapter 15 Synergizing sustainable green nanotechnology and AI/ML for advanced nanocarriers: A paradigm shift in the treatment of neurodegenerative diseases In: Koduru TS, Osmani RAM, Singh E, Dutta SBTTNR, Eds. The Neurodegeneration Revolution: Emerging Therapies and Sustainable Solutions Koduru T.S. Osmani R.A.M. Singh E. Dutta S.B.T.T.N.R. United States Academic Press 2025 373 397 10.1016/B978‑0‑443‑28822‑7.00017‑9
    [Google Scholar]
  6. Inamdar A. Gurupadayya B. Halagali P. Unraveling neurological drug delivery: Polymeric nanocarriers for enhanced blood-brain barrier penetration. Curr. Drug Targets 2024 26 1 24 39513304
    [Google Scholar]
  7. Chandra P. Ali Z. Fatima N. Sharma H. Sachan N. Sharma K.K. Shankhpushpi (Convolvulus pluricaulis): Exploring its cognitive enhancing mechanisms and therapeutic potential in neurodegenerative disorders. Curr. Bioact. Compd. 2024 21 2 E290424229510 10.2174/0115734072292339240416095600
    [Google Scholar]
  8. Lopresti A.L. Smith S.J. Majeed M. Drummond P.D. Effects of an oroxylum indicum extract (sabroxy®) on cognitive function in adults with self-reported mild cognitive impairment: A randomized, double-blind, placebo-controlled study. Front. Aging Neurosci. 2021 13 728360 10.3389/fnagi.2021.728360 34531736
    [Google Scholar]
  9. Sharma H. Pathak R. Biswas D. Unveiling the therapeutic potential of modern probiotics in addressing neurodegenerative disorders: A comprehensive exploration, review and future perspectives on intervention strategies. Curr Psychiatry Res Rev 2024 21 4 1 9 10.2174/0126660822304321240520075036
    [Google Scholar]
  10. Chandra P. Sharma H. Phosphodiesterase inhibitors for treatment of Alzheimer’s Disease. INDIAN DRUGS 2024 61 7 7 22 10.53879/id.61.07.14382
    [Google Scholar]
  11. Sharma H. Chandra P. Challenges and future prospects: A benefaction of phytoconstituents on molecular targets pertaining to alzheimer’s disease. Int. J. Pharm. Investig. 2023 14 1 117 126 10.5530/ijpi.14.1.15
    [Google Scholar]
  12. Sharma H. Chandra P. Verma A. Pandey S.N. Kumar P. Sigh A. Therapeutic approaches of nutraceuticals in the prevention of neurological disorders. Eur. Chem. Bull. 2023 12 5 1575 1596 10.48047/ecb/2023.12.si5a.038
    [Google Scholar]
  13. Dinda B. Mohanta B.C. Arima S. Sato N. Harigaya Y. Flavonoids from the stem-bark of Oroxylum indicum. Nat. Prod. Sci. 2007 13 3 190 194
    [Google Scholar]
  14. Al Noman A. Dev Sharma P. Jahin Mim T. Al Azad M. Sharma H. Molecular docking and ADMET analysis of coenzyme Q10 as a potential therapeutic agent for Alzheimer’s disease. Aging Pathobiol Ther 2024 6 4 1 13 10.31491/APT.2024.12.155
    [Google Scholar]
  15. Sharma H.C.P. Effects of natural remedies on memory loss and Alzheimer’s disease. Afr J Bio Sci 2024 6 7 187 211
    [Google Scholar]
  16. Summat R. Waiwut P. Daodee S. Phytomedicine potential of Oroxylum indicum root and its constituents: Targeting alzheimer’s disease. Plants 2025 14 2 223 10.3390/plants14020223 39861577
    [Google Scholar]
  17. Mairuae N. Buranrat B. Yannasithinon S. Cheepsunthorn P. Oroxylum indicum Kurz (L) leaf extract exerted antioxidant and anti-inflammatory effects on LPS-stimulated BV2 microglial cells. Trop. J. Pharm. Res. 2024 23 9 1409 1415 10.4314/tjpr.v23i9.2
    [Google Scholar]
  18. Sreedharan S. Pande A. Pande A. Majeed M. Cisneros-Zevallos L. The neuroprotective effects of Oroxylum indicum extract in SHSY-5Y neuronal cells by upregulating bdnf gene expression under lps induced inflammation. Nutrients 2024 16 12 1887 10.3390/nu16121887 38931243
    [Google Scholar]
  19. Othman F.A. Mat Zin A.A. Zakaria Y. Nik Salleh N.N.H. Mohd Satar A. Tan S.C. Pre-clinical acute oral toxicity and subacute neurotoxicity risk assessments on sprague dawley rats treated with single dose or repeated doses of flavonoid-enriched fraction extracted from Oroxylum indicum leaves. Drug Chem. Toxicol. 2025 ••• 1 17 10.1080/01480545.2024.2449210 39881662
    [Google Scholar]
  20. Singh K. Yadav A. Khan S. Baicalein isolated from Oroxylum indicum acts as a potent µ- and κ-opioid receptor antagonist agent via the reversal of agonist-mediated cAMP inhibition. Nat. Prod. Res. 2024 1 9 10.1080/14786419.2024.2396452
    [Google Scholar]
  21. Choudhury S. Dasmahapatra A.K. Destabilisation of Alzheimer’s amyloid-β protofibrils by Baicalein: Mechanistic insights from all-atom molecular dynamics simulations. Mol. Divers. 2024 1 17 10.1007/s11030‑024‑11001‑9 39379662
    [Google Scholar]
  22. Pondugula S.R. Majrashi M. Almaghrabi M. Oroxylum indicum ameliorates chemotherapy induced cognitive impairment. PLoS One 2021 16 6 e0252522 10.1371/journal.pone.0252522 34081735
    [Google Scholar]
  23. Dinda B. SilSarma I, Dinda M, Rudrapaul P. Oroxylum indicum (L.) Kurz, an important Asian traditional medicine: From traditional uses to scientific data for its commercial exploitation. J. Ethnopharmacol. 2015 161 255 278 10.1016/j.jep.2014.12.027 25543018
    [Google Scholar]
  24. Mairuae N. Connor J. Buranrat B. Lee S. Oroxylum indicum (L.) extract protects human neuroblastoma SH SY5Y cells against β amyloid induced cell injury. Mol. Med. Rep. 2019 20 2 1933 1942 10.3892/mmr.2019.10411 31257498
    [Google Scholar]
  25. Chaudhary A.K. Harminder, Singh V. A Review on the taxonomy, ethnobotany, chemistry and pharmacology of Oroxylum indicum vent. Indian J. Pharm. Sci. 2011 73 5 483 490 10.4103/0250‑474X.98981 22923859
    [Google Scholar]
  26. Rojsanga P. Bunsupa S. Sithisarn P. Flavones contents in extracts from oroxylum indicum seeds and plant tissue cultures. Molecules 2020 25 7 1545 10.3390/molecules25071545 32231034
    [Google Scholar]
  27. Barnes J. Trease and evans’ pharmacognosy. In:Focus on Alternative and Complementary Therapies. Amsterdam, Netherlands Elsevier Health Sciences 1999 151 152
    [Google Scholar]
  28. Rajkumar V. Guha G. Kumar R.A. Isolation and bioactivity evaluation of two metabolites from the methanolic extract of Oroxylum indicum stem bark. Asian Pac. J. Trop. Biomed. 2012 2 1 S7 S11 10.1016/S2221‑1691(12)60120‑8
    [Google Scholar]
  29. Pathak R. Sachan N. Kabra A. Isolation, characterization, development and evaluation of phytoconstituent based formulation for diabetic neuropathy. Saudi Pharm. J. 2023 31 8 101687 10.1016/j.jsps.2023.06.020 37448840
    [Google Scholar]
  30. Bhaumik S. Sarkar A. Debnath S. α-Glucosidase inhibitory potential of Oroxylum indicum using molecular docking, molecular dynamics, and in vitro evaluation. Saudi Pharm. J. 2024 32 6 102095 10.1016/j.jsps.2024.102095 38766274
    [Google Scholar]
  31. Ellman G.L. Courtney K.D. Andres V. Featherstone R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961 7 2 88 95 10.1016/0006‑2952(61)90145‑9 13726518
    [Google Scholar]
  32. Abubakar A. Olorunkemi M.R. Busari M.B. Hamzah R.U. Abdulrasheed-Adeleke T. Comparative in vitro antioxidant activities of aqueous extracts of garcinia kola and buchholzia coriacea seeds. Tanzan. J. Sci. 2020 46 2 498 507 10.4314/tjs.v46i2.25
    [Google Scholar]
  33. Imam M.Z. Akter S. Mazumder M.E.H. Rana M.S. Antioxidant activities of different parts of Musa sapientum L. ssp. sylvestris fruit. J. Appl. Pharm. Sci. 2011 1 10 68 72
    [Google Scholar]
  34. Rao S.B. Jayanthi M. Yogeetha R. Ramakrishnaiah H. Nataraj J. Free radical scavenging activity and reducing power of gnidia glauca(fresen.) gilg. J. Appl. Pharm. Sci. 2013 3 6 203 207 10.7324/JAPS.2013.3634
    [Google Scholar]
  35. Rahman M.M. Islam M.B. Biswas M. Khurshid Alam A.H.M. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res. Notes 2015 8 1 621 10.1186/s13104‑015‑1618‑6 26518275
    [Google Scholar]
  36. Jeremić S.R. Šehović S.F. Manojlović N.T. Marković Z.S. Antioxidant and free radical scavenging activity of purpurin. Monatsh. Chem. 2012 143 3 427 435 10.1007/s00706‑011‑0695‑z
    [Google Scholar]
  37. Chirumbolo S. Bjørklund G. Commentary: The flavonoid baicalein rescues synaptic plasticity and memory deficits in a mouse model of Alzheimer’s disease. Front. Neurol. 2016 7 AUG 141 10.3389/fneur.2016.00141 27618870
    [Google Scholar]
  38. Ali M. Muhammad S. Shah M.R. Neurologically potent molecules from Crataegus oxyacantha; isolation, anticholinesterase inhibition, and molecular docking. Front. Pharmacol. 2017 8 JUN 327 10.3389/fphar.2017.00327 28638340
    [Google Scholar]
  39. Ullah F. Ayaz M. Sadiq A. Phenolic, flavonoid contents, anticholinesterase and antioxidant evaluation of Iris germanica var; florentina. Nat. Prod. Res. 2016 30 12 1440 1444 10.1080/14786419.2015.1057585 26166432
    [Google Scholar]
  40. Inamdar A. Gurupadayya B. Halagali P. Cutting-Edge Strategies for Overcoming Therapeutic Barriers in Alzheimer’s Disease. Curr. Pharm. Des. 2025 31 8 598 618 39492772
    [Google Scholar]
  41. Sharma H. Tyagi S.J. Chandra P. Verma A. Kumar P. Ashique S. Role of exosomes in parkinson’s and alzheimer’s diseases. Mishra N. Ashique S. Garg A. Chithravel V. Anand K. Exosomes Based Drug Delivery Strategies for Brain Disorders. Singapore Springer 2024 147 182 10.1007/978‑981‑99‑8373‑5_6
    [Google Scholar]
  42. Kumar P. Sharma H. Singh A. Pandey S.N. Chandra P. Correlation between exosomes and neuro-inflammation in various brain disorders Exosomes Based Drug Delivery Strategies for Brain Disorders Mishra N. Ashique S. Garg A. Chithravel V. Anand K. Singapore Springer 2024 273 302 10.1007/978‑981‑99‑8373‑5_11
    [Google Scholar]
  43. Sharma H. Rachamalla H.K. Mishra N. Chandra P. Pathak R. Ashique S. Introduction to exosome and its role in brain disorders. Mishra N. Ashique S. Garg A. Chithravel V. Anand K. Exosomes Based Drug Delivery Strategies for Brain Disorders. Singapore Springer 2024 1 35 10.1007/978‑981‑99‑8373‑5_1
    [Google Scholar]
  44. Sarkar S. Bhui U. Kumar B. Ashique S. Kumar P. Sharma H. Correlation between cognitive impairment and peripheral biomarkers - significance of phosphorylated tau and amyloid-β in alzheimer ’ s disease: A new insight. Curr Psychiatry Res Rev 2024 1 25 10.2174/0126660822329981241007105405
    [Google Scholar]
  45. Xavier J. Reddy J. A comparative quantitative study on momordin in the fruit and leave extracts of two different cultivars of momordicacharantia linn. Int J Environ Agric Biotechnol 2017 2 6 2873 2880 10.22161/ijeab/2.6.13
    [Google Scholar]
  46. Singh T.G. Sharma A. Devi S. Exploring the mechanisms of chrysin in combating Alzheimer’s disease: Therapeutic perspectives. J. Appl. Pharm. Sci. 2024 14 5 69 78 10.7324/JAPS.2024.162341
    [Google Scholar]
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Neuroprotective Potential of Oroxylum indicum Seeds in Antioxidant Activity, Enzyme Inhibition and Cognitive Improvement for Alzheimer's Disease
Bentham Science Publishers ; https://doi.org/10.2174/0115734080365174250527061145
/content/journals/cei/10.2174/0115734080365174250527061145
/content/journals/cei/10.2174/0115734080365174250527061145
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  • Article Type:     Research Article
Keywords: Alzheimer’s disease ; chrysin ; Oroxylum indicum ; baicalein ; molecular docking ; antioxidant activity ; butyrylcholinesterase inhibition ; acetylcholinesterase inhibition

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