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image of Jinwei Guben Formula (Jinwei Formula II) Modulates Neutrophil Extracellular Trap Formation and Enhances Glucocorticoid Efficacy in COPD Through HDAC2 Regulation
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Abstract

Introduction

Neutrophil extracellular traps (NETs) contribute to chronic inflammation in chronic obstructive pulmonary disease (COPD). Jinwei Guben Formula (JWGB) is a clinically used TCM for COPD, but its mechanism regarding NETs and glucocorticoid sensitivity is unclear. This study aimed to investigate whether JWGB modulates NETs formation and glucocorticoid efficacy HDAC2 in COPD.

Methods

An integrated approach combining UHPLC-MS/MS, network pharmacology, molecular docking, and human neutrophil experiments was used.

Results

We identified 168 compounds in JWGB. Network analysis predicted 42 active ingredients and highlighted the NETs formation pathway. Key ingredients showed strong binding to HDAC2 and MPO. Experimentally, JWGB inhibited CSE-induced NETs release and inflammation. Notably, JWGB upregulated HDAC2 expression in COPD neutrophils and synergized with methylprednisolone, allowing a low dose (100 µM) to match the efficacy of a high dose (500 µM).

Discussion

Our findings reveal that JWGB alleviates COPD by simultaneously inhibiting NET formation and restoring HDAC2 function. This dual action provides a mechanistic basis for its clinical efficacy and suggests its potential as a steroid-sparing strategy to overcome glucocorticoid resistance.

Conclusion

JWGB may alleviate neutrophilic inflammation in COPD by inhibiting NET formation and upregulating HDAC2. Its synergy with glucocorticoids suggests potential as a steroid-sparing therapy.

© 2026 The Author(s). Published by Bentham Science Publishers.
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2026-02-11
2026-02-24

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Jinwei Guben Formula (Jinwei Formula II) Modulates Neutrophil Extracellular Trap Formation and Enhances Glucocorticoid Efficacy in COPD Through HDAC2 Regulation
Bentham Science Publishers ; https://doi.org/10.2174/0115734099441592251222045407
/content/journals/cad/10.2174/0115734099441592251222045407
/content/journals/cad/10.2174/0115734099441592251222045407
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  • Article Type:     Research Article
Keywords: COPD ; glucocorticoids ; jinwei guben formula ; molecular docking ; network pharmacology ; HDAC2 ; neutrophil extracellular traps

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