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2000
Volume 24, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Introduction

Ciprofloxacin hydrochloride (CH) is a widely used fluoroquinolone antibiotic. Accurate, cost-effective methods are essential for its routine quality control. This study aimed to develop and validate simple UV spectrophotometric and spectrofluorimetric methods for quantifying CH in bulk and pharmaceutical formulations.

Methods

UV analysis was performed at 271 nm, while fluorescence measurements were taken at 335 nm (excitation) and 416 nm (emission) in phosphate buffer (pH 7). Validation was conducted in accordance with the International Council for Harmonisation (ICH) guidelines for linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ), robustness, and solution stability. Results were compared with an official HPLC method.

Results

Both methods demonstrated excellent linearity in the range of 0.4-2.0 × 10-5 M (r2 = 0.9999). The limits of detection (LOD) and quantification (LOQ) were 0.64/1.95 × 10-6 M for the UV method and 0.66/1.99 × 10-6 M for the fluorescence method. Recovery values ranged from 99.27% to 99.92% (UV) and 98.60% to 100.32% (fluorescence), with %RSD values below 2%, indicating good precision. Robustness testing revealed minimal effects from deliberate variations in experimental conditions, and the results showed strong agreement with the compendial HPLC method.

Discussion

UV spectrophotometric and spectrofluorimetric methods proved to be accurate, sensitive, and reproducible. The UV method is ideal for basic lab setups, while fluorescence offers greater sensitivity for lower concentrations. Comparable results with HPLC confirm their suitability.

Conclusion

Validated UV and fluorescence methods provide efficient, accurate, and low-cost alternatives to HPLC for quality control of CH in pharmaceutical analysis.

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/content/journals/aia/10.2174/0122113525386386250704103943
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Spectrofluorimetric and Spectrophotometric Methods for the Determination of Ciprofloxacin HCl and Their Comparison with the Compendial High-Performance Liquid Chromatography (HPLC) Method
Anti Infect. Agents 24, E22113525386386 (2026); https://doi.org/10.2174/0122113525386386250704103943
/content/journals/aia/10.2174/0122113525386386250704103943
/content/journals/aia/10.2174/0122113525386386250704103943
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  • Article Type:     Research Article
Keyword(s): ciprofloxacin HCl; Fluoroquinolones; HPLC; ICH guidelines; spectrofluorimetry; spectrophotometry

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