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2000
Volume 24, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Introduction

Surgical site infections (SSIs) are the most common surgery related complication in healthcare worldwide. Infections due to carbapenem resistant are increasing dramatically with significant impact on surgical patients.

Objective

The aim of this study was to detect and efflux pump genes in isolated from infected surgical wounds.

Methods

A study including 291 patients was conducted at Birat Medical College Teaching Hospital (BMCTH), Biratnagar, from October 2021 to October 2022. Specimens were collected from the patients with SSIs under aseptic conditions and sent to the microbiology laboratory. was identified by VITEK 2. The presence of carbapenemase was phenotypically screened by the modified carbapenem inactivation method (mCIM). and efflux pump genes were detected by polymerase chain reaction (PCR).

Results and Discussion

A total of 134 (46%) specimens were culture positive, out of which 24 (30%) were identified as with 11 (45.8%) isolates resistant to carbapenem. Tigecycline and colistin were the most effective drugs against . Among these isolates, seven were found to be mCIM positive. was absent in all mCIM-positive isolates, however, efflux pump genes ( and ) were detected in all of the carbapenem-resistant isolates.

Conclusion

A high prevalence of carbapenem-resistant could be attributed to the efflux pump genes. This finding suggests that the AcrAB efflux pump may be the major virulence factor that can contribute to multidrug-resistant strains.

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2026-03-09

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blaKPC and Efflux Pump Genes in Klebsiella pneumoniae Isolated from Surgical Site Infections
Anti Infect. Agents 24, E22113525347585 (2026); https://doi.org/10.2174/0122113525347585250414063103
/content/journals/aia/10.2174/0122113525347585250414063103
/content/journals/aia/10.2174/0122113525347585250414063103
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  • Article Type:     Research Article
Keyword(s): Carbapenem; carbapenemase; efflux pump; modified carbapenem inactivation method; polymerase chain reaction; VITEK 2

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